SD2 - Andor Spinning Disc FRAPPA
From BioDIP
(Difference between revisions)
(Dual View taken out, software version upgrade to 2.4) |
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|ill7=Laser Diode 405 nm (pulsed) | |ill7=Laser Diode 405 nm (pulsed) | ||
|detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; pixel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.39x (was 2.067x before addition of the dual filter wheel Apr 28th 2011 (was 1.9x before addition of dual camera switch)) in front of iXon or a 1.28x (was 1.2x before addition of the dual filter wheel Apr 28th 2011) in front of Clara<br>image pixel size for use with iXon (measured): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) <br> pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px | |detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; pixel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.39x (was 2.067x before addition of the dual filter wheel Apr 28th 2011 (was 1.9x before addition of dual camera switch)) in front of iXon or a 1.28x (was 1.2x before addition of the dual filter wheel Apr 28th 2011) in front of Clara<br>image pixel size for use with iXon (measured): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) <br> pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px | ||
| − | |reflectors= | + | |reflectors=Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) |
|features=- | |features=- | ||
| − | |software=iQ 1.10.5 [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:IQ_1_10_2_User_Guide.pdf user guide] + iQ 2. | + | |software=iQ 1.10.5 [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:IQ_1_10_2_User_Guide.pdf user guide] + iQ 2.4 (with Python) |
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA | |link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA | ||
Revision as of 17:04, 4 January 2012
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor Dual camera port with two cameras: Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um Andor Clara cooled interline CCD; pixel size of chip: 6.45um note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.39x (was 2.067x before addition of the dual filter wheel Apr 28th 2011 (was 1.9x before addition of dual camera switch)) in front of iXon or a 1.28x (was 1.2x before addition of the dual filter wheel Apr 28th 2011) in front of Clara image pixel size for use with iXon (measured): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px | ||
| reflectors | Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | - | ||
| software | [[software::iQ 1.10.5 user guide + iQ 2.4 (with Python)]] | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
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| inv.nr. | 0 10.1.145.20 |
