SD2 - Andor Spinning Disc FRAPPA
From BioDIP
(Difference between revisions)
(update of values for camera projecting lenses in front of Clara and iXon, moved old value to end of section) |
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|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
|ill7=Laser Diode 405 nm (pulsed) | |ill7=Laser Diode 405 nm (pulsed) | ||
| − | |detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; pixel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.475x | + | |detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; pixel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.475x in front of iXon or a 1.285x in front of Clara<br>image pixel size for use with iXon (measured): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) <br> pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px <br> old values: <br>projecting lens in front of iXon: was 2.39x before removal of DualView on Dec 21st 2011; was 2.067x before addition of the dual filter wheel on Apr 28th 2011, was 1.9x before addition of dual camera switch on June 30th 2010 <br> projecting lens in front of Clara: was 1.28x before removal of DualView on Dec 21st 2011; was 1.2x before addition of the dual filter wheel on Apr 28th 2011 |
|reflectors=Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | |reflectors=Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
|features=- | |features=- | ||
Revision as of 17:15, 4 January 2012
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor Dual camera port with two cameras: Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um Andor Clara cooled interline CCD; pixel size of chip: 6.45um note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.475x in front of iXon or a 1.285x in front of Clara image pixel size for use with iXon (measured): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px old values: projecting lens in front of iXon: was 2.39x before removal of DualView on Dec 21st 2011; was 2.067x before addition of the dual filter wheel on Apr 28th 2011, was 1.9x before addition of dual camera switch on June 30th 2010 projecting lens in front of Clara: was 1.28x before removal of DualView on Dec 21st 2011; was 1.2x before addition of the dual filter wheel on Apr 28th 2011 | ||
| reflectors | Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | - | ||
| software | [[software::iQ 1.10.5 user guide + iQ 2.4 (with Python)]] | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
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| inv.nr. | 0 10.1.145.20 |
