SD2 - Andor Spinning Disc FRAPPA
From BioDIP
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| system-name = Andor Spinning Disc FRAPPA | | system-name = Andor Spinning Disc FRAPPA | ||
| category = Real-Time Confocal | | category = Real-Time Confocal | ||
| − | | stand = inverted, | + | | stand = inverted, motorized, 2x optovar |
| image = MPI19.jpg | | image = MPI19.jpg | ||
| facility = MPI-CBG LMF | | facility = MPI-CBG LMF | ||
| Line 10: | Line 10: | ||
| room = 038B | | room = 038B | ||
| description = fast, "real-time" confocal imaging | | description = fast, "real-time" confocal imaging | ||
| − | | applications = confocal imaging of EGFP | + | | applications = confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution. |
| − | | microscope = Olympus | + | | microscope = Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage |
| − | | objectives = 10x / 0. | + | | objectives = 10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br>100x / 1.35 Oil UPlanSApo |
| − | | illumination = | + | | illumination = Cairn OptoLED lite<br>HBO<br>405nm diode laser<br>488 nm solid-state laser Coherent Sapphire<br>561 nm solid-state laser Cobolt Jive<br>640nm solid-state laser |
| − | | detection = Spinning disc scan head Yokogawa | + | | detection = Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD<br> |
| − | | reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS | + | | reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: empty |
| − | | features = | + | | features = Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant |
| incubation = stage incubator | | incubation = stage incubator | ||
| link1 = [http://spreadsheets.google.com/pub?key=pFACvabR4mSkKjMo69HOPSg LPM] | | link1 = [http://spreadsheets.google.com/pub?key=pFACvabR4mSkKjMo69HOPSg LPM] | ||
Revision as of 15:52, 14 January 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , inverted, motorized, 2x optovar stand, Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
| objectives |
| ||
| illumination |
| ||
| detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: empty | ||
| features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
| software | |||
| incubation | stage incubator | ||
| links |
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| inv.nr. |
[[Category:{{{type}}}]]
