SD2 - Andor Spinning Disc FRAPPA
From BioDIP
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| illumination = Cairn OptoLED lite<br>HBO<br>405nm diode laser<br>488 nm solid-state laser Coherent Sapphire<br>561 nm solid-state laser Cobolt Jive<br>640nm solid-state laser | | illumination = Cairn OptoLED lite<br>HBO<br>405nm diode laser<br>488 nm solid-state laser Coherent Sapphire<br>561 nm solid-state laser Cobolt Jive<br>640nm solid-state laser | ||
| detection = Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD<br> | | detection = Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD<br> | ||
| − | | reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube | + | | reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) |
| features = Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | | features = Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | ||
| incubation = stage incubator | | incubation = stage incubator | ||
Revision as of 15:29, 29 January 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , inverted, motorized, 2x optovar stand, Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
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| detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
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| incubation | stage incubator | ||
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