SD2 - Andor Spinning Disc FRAPPA
From BioDIP
(Difference between revisions)
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| applications = confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution. | | applications = confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution. | ||
| microscope = Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage | | microscope = Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
| − | | objectives = 10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br> | + | | objectives = 10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br>60x / 1.35 Oil UPlanSApo |
| − | | illumination = Cairn OptoLED lite<br>HBO<br>405nm diode laser<br>488 nm solid-state laser Coherent Sapphire<br>561 nm solid-state laser Cobolt Jive<br>640nm solid-state laser | + | | illumination = Cairn OptoLED lite<br>HBO<br>405nm diode laser, 50mW<br>488 nm solid-state laser Coherent Sapphire, 50mW<br>561 nm solid-state laser Cobolt Jive, 50mW<br>640nm solid-state laser, 40mW |
| − | | detection = Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD<br> | + | | detection = Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU<br>image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um <br> |
| reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | | reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features = Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | | features = Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | ||
Revision as of 14:27, 7 May 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , inverted, motorized, 2x optovar stand, Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
| objectives |
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| illumination |
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| detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
| software | |||
| incubation | stage incubator | ||
| links |
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| inv.nr. |
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