SD2 - Andor Spinning Disc FRAPPA
From BioDIP
(Difference between revisions)
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|obj2=Olympus UPlanSApo 60x 1.20 W | |obj2=Olympus UPlanSApo 60x 1.20 W | ||
|obj3=Olympus UPlanSApo 60x 1.35 Oil | |obj3=Olympus UPlanSApo 60x 1.35 Oil | ||
+ | |obj4=Olympus UPlanSApo 40X 0.9 | ||
|ill1=Transmitted Light (LED) | |ill1=Transmitted Light (LED) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
Line 23: | Line 24: | ||
|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
|ill7=Laser Diode 405 nm (pulsed) | |ill7=Laser Diode 405 nm (pulsed) | ||
− | |detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: | + | |detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: 0.25um, back-projected pinhole spacing: 2.54um (with 60x objective, 1x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU<br>image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um <br> |
− | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: | + | |reflectors=Optical Insight DualView image splitter: <br> insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting<br>insert with T565 lpxr DC and empty EM position<br>insert for CFP/YFP splitting<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) |
|features=Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | |features=Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater |
Revision as of 19:12, 24 September 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | , Inverted stand, motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
objectives | |||
illumination | |||
detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: 0.25um, back-projected pinhole spacing: 2.54um (with 60x objective, 1x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um | ||
reflectors | Optical Insight DualView image splitter: insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting insert with T565 lpxr DC and empty EM position insert for CFP/YFP splitting Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
software | |||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 |