SD2 - Andor Spinning Disc FRAPPA
From BioDIP
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.0x before and 1.9x after the CSU image pixel size (calculated): with 60x objective: 0.14um; 60x obj. and 2.0x optovar: 0.07um; 40x obj.: 0.21um; 40x obj. and 2.0x opt.: 0.11um | ||
reflectors | Optical Insight DualView image splitter: insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting insert with T565 lpxr DC and empty EM position insert for CFP/YFP splitting Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
software | [[software::iQ 1.10.2 user guide]] | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 |