SD2 - Andor Spinning Disc FRAPPA

Suitable for confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution..

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	, inverted, motorized, 2x optovar stand, Olympus IX81 with ZDC (hardware autofocus), Prior NanoScanZ, Prior ProScanII xy scanning stage
objectives
illumination
detection	Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um
reflectors	Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40)
features	Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki.
software
incubation	stage incubator
links	[ manual] [LPM laser power values] [- beam path] [ advanced system check]
inv.nr.

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