SD2 - Andor Spinning Disc FRAPPA

Suitable for confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	, Inverted stand, motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage
objectives	Olympus UPlanSApo 10x 0.4 Olympus UPlanSApo 60x 1.20 W Olympus UPlanSApo 60x 1.35 Oil
illumination	Transmitted Light (LED) Fluorescence (HBO) Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm Laser Diode 405 nm (pulsed)
detection	Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um
reflectors	Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40)
features	Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki.
software
incubation	Stage incubator (T) + objective heater
links	[ manual] laser power values [- beam path] [ advanced system check]
inv.nr.	0

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