SD2 - Andor Spinning Disc FRAPPA

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage
objectives	Olympus UPlanSApo 10x 0.4 Olympus UPlanSApo 60x 1.20 W Olympus UPlanSApo 60x 1.35 Oil Olympus UPlanSApo 40X 0.9
illumination	Transmitted Light (LED) Fluorescence (HBO) Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm Laser Diode 405 nm (pulsed)
detection	Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.0x before and 1.9x after the CSU image pixel size (calculated): with 60x objective: 0.14um; 60x obj. and 2.0x optovar: 0.07um; 40x obj.: 0.21um; 40x obj. and 2.0x opt.: 0.11um
reflectors	Optical Insight DualView image splitter: insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting insert with T565 lpxr DC and empty EM position insert for CFP/YFP splitting Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40)
features	Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki.
software	[[software::iQ 1.10.2 user guide]]
incubation	Stage incubator (T) + objective heater
links	[ manual] laser power values [- beam path] [ advanced system check]
inv.nr.	0

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