SD2 - Andor Spinning Disc FRAPPA

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage
objectives	Olympus UPlanSApo 10x 0.4 Olympus UPlanSApo 60x 1.20 W Olympus UPlanSApo 60x 1.35 Oil Olympus UPlanSApo 40X 0.9 Olympus UPlanSApo 100x 1.4 Oil Olympus UPlanSApo 20x 0.75 0.65mm WD
illumination	Transmitted Light (LED) Fluorescence (HBO) Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm Laser Diode 405 nm (pulsed)
detection	Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor Dual camera port with two cameras: Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um Andor Clara cooled interline CCD; pixel size of chip: 6.45um note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.39x (was 2.067x before addition of the dual filter wheel Apr 28th 2011 (was 1.9x before addition of dual camera switch)) in front of iXon or a 1.28x (was 1.2x before addition of the dual filter wheel Apr 28th 2011) in front of Clara image pixel size for use with iXon (measured): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px
reflectors	Optical Insight DualView image splitter: insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting insert with T565 lpxr DC and empty EM position insert for CFP/YFP splitting Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40)
features	-
software	[[software::iQ 1.10.5 user guide + iQ 2.3 (with Python)]]
incubation	Stage incubator (T) + objective heater
links	[ manual] laser power values [- beam path] [ advanced system check]
inv.nr.	0

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