SP5 I, upright, BIOTEC
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO15 |
| − | |system-name= | + | |system-name=SP5 I, upright, BIOTEC |
|type=Microscope | |type=Microscope | ||
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
| − | |building= | + | |building=BIOTEC |
|room=220 | |room=220 | ||
| − | |description=Our [[Leica TCS SP5]] I [[confocal]] | + | |Facility-logo=Imaging@Biotec.jpg |
| + | |Institute-logo=Biotec logo.png | ||
| + | |description=Our [[Leica TCS SP5]] I [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | ||
|applications=- | |applications=- | ||
|image=Bioz02.JPG | |image=Bioz02.JPG | ||
| − | |stand-name=Leica - | + | |stand-name=Leica - DM6000 |
| − | |stand= | + | |stand=upright |
|microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | ||
|obj1=Leica HCX PL APO 40x 0.75 | |obj1=Leica HCX PL APO 40x 0.75 | ||
|obj2=Leica HC PL APO 20x 0.7 | |obj2=Leica HC PL APO 20x 0.7 | ||
|obj3=Leica HC PL APO 10x 0.4 | |obj3=Leica HC PL APO 10x 0.4 | ||
| − | | | + | |obj5=Leica HC PL FLUOTAR 5x 0.15 |
| − | | | + | |obj6=Leica HC PL APO CS 40x 1,25-0,75 Oil |
| − | | | + | |obj7=Leica HC PL APO 100x 1.4 Oil |
| − | | | + | |obj8=Leica HCX PL APO 100x 1.47 Oil Korr. |
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
| − | |ill2=Fluorescence ( | + | |ill2=Fluorescence (Metal Halide, HXP, 120W) |
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
|ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | |ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | ||
|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser HeNe 633 nm | |ill6=Laser HeNe 633 nm | ||
| − | |detection=point scanner, | + | |detection=point scanner, 2 confocal HyD´s, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole |
| − | |reflectors=FITC ( | + | |reflectors=GFP/FITC (L5) : EX 480/40 ; BS 505 ; EM BP 527/30<br> |
| + | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br> | ||
| + | TRITC/DsRed/RFP : EX 545/30 ; LP 570 ; EM BP 620/60<br> | ||
| + | Analysator | ||
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | ||
| − | |software= | + | |software=LAS AF |
| − | |incubation= | + | |incubation=Cage incubator (T+CO2) |
| − | | | + | |link0=http://www.biotec.tu-dresden.de/fileadmin/technology/imaging/Leica_SP5_upright_I_-_manual.pdf |
| + | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_SP5I | ||
|link2=- | |link2=- | ||
| − | |inv= | + | |inv=146330 |
|objectives=10x/0.4<br>20x/0.7 DIC<br>40x/0.75 DIC<br>20x/0,5 W (Dipping) DIC<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | |objectives=10x/0.4<br>20x/0.7 DIC<br>40x/0.75 DIC<br>20x/0,5 W (Dipping) DIC<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | ||
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | ||
}} | }} | ||
Latest revision as of 12:46, 22 May 2015
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Leica - DM6000, upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | ||
| objectives | |||
| illumination | |||
| detection | point scanner, 2 confocal HyD´s, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
| reflectors | GFP/FITC (L5) : EX 480/40 ; BS 505 ; EM BP 527/30 DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | ||
| software | LAS AF | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 146330 |
