SP5 I, upright, BIOTEC
From BioDIP
(Difference between revisions)
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=02 |
| − | | system-name | + | |system-name=Sp5 I |
| − | | category | + | |type=Microscope |
| − | | | + | |category=Laser Scanning Confocal |
| − | | | + | |facility=Imaging@Biotec |
| − | | | + | |building=Biotec |
| − | | facility_image | + | |room=220 |
| − | + | |facility_image=logo-imagingatbiotec.jpg | |
| − | + | |description=Our [[Leica TCS SP5]] I [[confocal]] system is designed for routine work and dedicated for high resolution imaging in XYZ. The upright confoguration and the dipping lenses make it optimal for embryo development studies. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | |
| − | | description | + | |applications=- |
| − | | applications | + | |image=Bioz02.JPG |
| − | | | + | |stand=Upright |
| − | | | + | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC |
| − | | illumination | + | |obj1=Leica HC PL APO 10x 0.4 |
| − | | detection | + | |obj2=Leica HC PL APO 20x 0.7 |
| − | | reflectors | + | |obj3=- |
| − | | features | + | |obj4=- |
| − | | incubation | + | |obj5=Leica HCX APO 63x 0.9 W |
| − | | link1 | + | |obj6=Leica HCX PL APO 63x 1.2 W |
| − | | link2 | + | |obj7=- |
| − | | inv | + | |obj8=- |
| + | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | ||
| + | |detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
| + | |reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515<br>BGR Triple DAPI GFP DsRed : EX 420/30 495/15 570/20 ; BS 455 510 590 ; EM 465/20 530/30 640/40 | ||
| + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | ||
| + | |incubation=- | ||
| + | |link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNuFT702uqALWA LPM] | ||
| + | |link2=- | ||
| + | |inv=- | ||
| + | |objectives=10x/0.4<br>20x/0.7 DIC<br>40x/0.75 DIC<br>20x/0,5 W (Dipping) DIC<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | ||
}} | }} | ||
Revision as of 14:07, 4 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | , Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | ||
| objectives | |||
| illumination |
| ||
| detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
| reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515 BGR Triple DAPI GFP DsRed : EX 420/30 495/15 570/20 ; BS 455 510 590 ; EM 465/20 530/30 640/40 | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | ||
| software | |||
| incubation | - | ||
| links |
| ||
| inv.nr. | - |
