| system-number       = 03

| system-name         = Sp5 II

| category             = Laser Scanning Confocal

| stand                = upright

| image                = Bioz03.JPG

| facility            = Imaging@Biotec

| facility_image      = logo-imagingatbiotec.jpg

| building            = Biotec

| description         = Our Leica SP5 II is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. A resonating scanner allows fast confocal imaging. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

| microscope          = upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional)

| objectives          = 10x/0.4 DIC<br>10x/0.3 W (Dipping)<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil<br>40x/0.8 W (Dipping)<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC

| illumination        = HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe

| detection           = point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole

| reflectors           = DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP

| features             = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner

| incubation           = Temperature control, CO2 control on requirement

| link1                = [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNton-D7Mn5CRw LPM]

| link2               = -