SP5 II, upright, CRTD
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{{Equipment | {{Equipment | ||
− | |system-number= | + | |system-number=BIO16 |
− | |system-name= | + | |system-name=SP5 II, upright, CRTD |
|type=Microscope | |type=Microscope | ||
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
− | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
− | |building= | + | |building=BIOTEC |
|room=220 | |room=220 | ||
− | | | + | |Facility-logo=Imaging@Biotec.jpg |
− | | | + | |Institute-logo=Biotec logo.png |
− | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples | + | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. |
|applications=- | |applications=- | ||
− | |image= | + | |image=SP5 II, upright, CRTD.jpg |
− | |stand-name=Leica - | + | |stand-name=Leica - DM6000 |
|stand=Upright | |stand=Upright | ||
− | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | + | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC |
− | |obj1=Leica | + | |obj1=Leica HC PL APO CS 10x 0.4 |
− | |obj2=Leica | + | |obj2=Leica HC PL APO CS 20x 0.7 |
− | |obj3=Leica HCX APO | + | |obj3=Leica HCX PL APO 40x 0.75 |
− | |obj4=Leica HCX APO 63x | + | |obj4=Leica HCX PL APO 63x 1.2 W |
− | |obj5=Leica HC PL APO | + | |obj5=Leica HC PL APO 100x 1.4 Oil |
− | |obj6=Leica | + | |obj6=Leica HCX APO L 20x 0.5 W |
− | |obj7=Leica HCX | + | |obj7=Leica HCX APO L 40x 0.8 W |
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
− | |ill2=Fluorescence ( | + | |ill2=Fluorescence (Metal Halide, HXP, 120W) |
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
|ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | |ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | ||
Line 29: | Line 29: | ||
|ill6=Laser HeNe 633 nm | |ill6=Laser HeNe 633 nm | ||
|detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | |detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
− | |reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 | + | |reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br> |
− | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | + | GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40<br> |
− | |software= | + | TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590<br> |
+ | Analysator | ||
+ | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
+ | |software=LAS AF | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
− | | | + | |link0=http://www.biotec.tu-dresden.de/fileadmin/technology/imaging/Leica_SP5_upright_II_-_manual.pdf |
+ | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_SP5II_upright | ||
|link2=- | |link2=- | ||
− | |inv= | + | |inv=71927 |
+ | |facility_image=Imaging@Biotec.jpg | ||
+ | |logo_image=LOGO_CRTD.jpg | ||
|objectives=10x/0.4 DIC<br>10x/0.3 W (Dipping)<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil<br>40x/0.8 W (Dipping)<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | |objectives=10x/0.4 DIC<br>10x/0.3 W (Dipping)<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil<br>40x/0.8 W (Dipping)<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | ||
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | ||
}} | }} |
Latest revision as of 11:05, 2 November 2017
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
microscope | Leica - DM6000, Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | ||
objectives | |||
illumination | |||
detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40 | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
software | LAS AF | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. | 71927 |