SP5 II, upright, CRTD
From BioDIP
(Difference between revisions)
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=03 |
| − | | system-name | + | |system-name=Sp5 II |
| − | | category | + | |type=Microscope |
| − | | | + | |category=Laser Scanning Confocal |
| − | | | + | |facility=Imaging@Biotec |
| − | | | + | |building=Biotec |
| − | | facility_image | + | |room=220 |
| − | + | |facility_image=logo-imagingatbiotec.jpg | |
| − | + | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. A resonating scanner allows fast confocal imaging. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | |
| − | | description | + | |applications=- |
| − | | applications | + | |image=Bioz03.JPG |
| − | | | + | |stand=Upright |
| − | | | + | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional) |
| − | | illumination | + | |obj1=Leica HC PL APO 10x 0.4 |
| − | | detection | + | |obj2=- |
| − | | reflectors | + | |obj3=Leica HC PL APO 20x 0.7 |
| − | | features | + | |obj4=Leica HCX PL APO 40x 1.25-0.75 Oil |
| − | | incubation | + | |obj5=- |
| − | | link1 | + | |obj6=Leica HCX APO 63x 0.9 W |
| − | | link2 | + | |obj7=Leica HCX PL APO 63x 1.2 W |
| − | | inv | + | |obj8=- |
| + | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | ||
| + | |detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
| + | |reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP | ||
| + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner | ||
| + | |incubation=cage incubator (T+CO2) | ||
| + | |link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNton-D7Mn5CRw | ||
| + | |link2=- | ||
| + | |inv=- | ||
| + | |objectives=10x/0.4 DIC<br>10x/0.3 W (Dipping)<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil<br>40x/0.8 W (Dipping)<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | ||
}} | }} | ||
Revision as of 14:10, 4 September 2009
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | , Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional) | ||
| objectives | |||
| illumination |
| ||
| detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
| reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515 TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner | ||
| software | |||
| incubation | cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | - |
