SP5 II, upright, CRTD
From BioDIP
(Difference between revisions)
Line 7: | Line 7: | ||
|building=Biotec | |building=Biotec | ||
|room=220 | |room=220 | ||
− | | | + | |Facility-logo=Imaging@Biotec.jpg |
− | | | + | |Institute-logo=LOGO CRTD.jpg |
|description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. A resonating scanner allows fast confocal imaging. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. A resonating scanner allows fast confocal imaging. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | ||
|applications=- | |applications=- | ||
Line 36: | Line 36: | ||
|link2=- | |link2=- | ||
|inv=- | |inv=- | ||
+ | |facility_image=Imaging@Biotec.jpg | ||
+ | |logo_image=LOGO_CRTD.jpg | ||
|objectives=10x/0.4 DIC<br>10x/0.3 W (Dipping)<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil<br>40x/0.8 W (Dipping)<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | |objectives=10x/0.4 DIC<br>10x/0.3 W (Dipping)<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil<br>40x/0.8 W (Dipping)<br>63x/0.9 W (Dipping)<br>63x/1.2 W Corr DIC | ||
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe | ||
}} | }} |
Revision as of 17:27, 26 November 2010
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
microscope | Leica - DMRE, Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional) | ||
objectives | |||
illumination | |||
detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP Analysator empty GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40 | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner | ||
software | - | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. | - |