SP5 II, upright, CRTD

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|building=Biotec
 
|building=Biotec
 
|room=220
 
|room=220
|facility_image=logo-imagingatbiotec.jpg
+
|facility_image=Imaging@Biotec.jpg
 
|description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. A resonating scanner allows fast confocal imaging. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. A resonating scanner allows fast confocal imaging. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|applications=-
 
|applications=-
 
|image=Bioz03.JPG
 
|image=Bioz03.JPG
 +
|stand-name=Leica - DMRE
 
|stand=Upright
 
|stand=Upright
 
|microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional)
 
|microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional)
Line 29: Line 30:
 
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP<br>Analysator<br>empty<br>GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40
 
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP<br>Analysator<br>empty<br>GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40
 
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner
 
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner
 +
|software=-
 
|incubation=Cage incubator (T+CO2)
 
|incubation=Cage incubator (T+CO2)
 
|link1=http://spreadsheets.google.com/ccc?key=pMZJXCgd5HNton-D7Mn5CRw
 
|link1=http://spreadsheets.google.com/ccc?key=pMZJXCgd5HNton-D7Mn5CRw

Revision as of 22:18, 19 November 2010

Suitable for -.
Bioz03.JPG


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. ("Biotec" is not in the list of possible values (BIOTEC, CRTD, DZNE, MTZ, MPI-CBG) for this property., 220)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Leica - DMRE, Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional)
objectives
Leica HCX PL APO 63x 1.2 W
Leica HCX APO L 20x 0.5 W
Leica HCX APO L 40x 0.8 W
Leica HCX APO 63x 0.9 W
Leica HC PL APO 10x 0.4
Leica HC PL APO 20x 0.7
Leica HCX PL APO 40x 0.75
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 496, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
detection point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole
reflectors DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425
TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP
Analysator
empty
GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP, resonating scanner
software -
incubation Cage incubator (T+CO2)
links
  • [- beam path]
  • [ advanced system check]
inv.nr. -



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