SP5 II, upright, CRTD
From BioDIP
(Difference between revisions)
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|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
− | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples | + | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. |
|applications=- | |applications=- | ||
|image=SP5 II, upright, CRTD.jpg | |image=SP5 II, upright, CRTD.jpg | ||
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TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590<br> | TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590<br> | ||
Analysator | Analysator | ||
− | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP |
|software=LAS AF | |software=LAS AF | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) |
Revision as of 12:08, 24 January 2013
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
microscope | Leica - DM6000, Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional) | ||
objectives | |||
illumination | |||
detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40 | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
software | LAS AF | ||
incubation | Cage incubator (T+CO2) | ||
links |
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inv.nr. | 71927 |