Technical Information

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= Light Microscopy =
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== Confocal Microscopy ==
 
== Confocal Microscopy ==
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/confocal.pdf Confocal Principle]<br>The document shows a simplified beam path and the basic compounds of an inverted Confocal Laser Scanning Microscope (CLSM).
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beads.mov Effect of the confocal pinhole]<br>This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.<br>The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization.
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=== Beam Paths ===
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_leica-sp5-aobs.pdf Leica TCS SP5 AOBS]
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_leica-spe.pdf Leica TCS SPE]
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_leica-sp2-aobs.pdf Leica TCS SP2 AOBS]
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_nikon-a1r.pdf Nikon A1R]
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_olympus-fv1000.pdf Olympus FV1000]
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_zeiss-710.pdf Zeiss LSM 710]
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_zeiss-510-meta.pdf Zeiss LSM 510 Meta]
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=== Laser Modules ===
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* Zeiss LSM 510<br>[[image:01_zeiss_lsm_510_lasermodule.jpg|150px]]
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* Leica TCS SP5<br>[[image:02_leica_tcs_sp5_lasermodule.jpg|150px]]

Latest revision as of 16:40, 24 June 2008

Contents

[edit] Light Microscopy

[edit] Confocal Microscopy

[edit] Beam Paths

[edit] Laser Modules

  • Zeiss LSM 510
    01 zeiss lsm 510 lasermodule.jpg
  • Leica TCS SP5
    02 leica tcs sp5 lasermodule.jpg
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