Technical Information

Revision as of 15:34, 23 May 2008

Confocal Principle
The document shows a simplified beam path and the basic compounds of an inverted Confocal Laser Scanning Microscope (CLSM).
Effect of the confocal pinhole
This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.
The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization.

@@ Line 1: / Line 1: @@
 == Confocal Microscopy ==
 * [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/confocal.pdf Confocal Principle]<br>The document shows a simplified beam path and the basic compounds of an inverted Confocal Laser Scanning Microscope (CLSM).
-* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beads.mov Effect of the confocal pinhole]<br># This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.<br>The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization.
+* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beads.mov Effect of the confocal pinhole]<br>This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.<br>The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization.