Technical Information
From BioDIP
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* [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beads.mov Effect of the confocal pinhole]<br>This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.<br>The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization. | * [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beads.mov Effect of the confocal pinhole]<br>This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.<br>The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization. | ||
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| + | === Beam Paths === | ||
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| + | * [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/technical_files/beampaths/beampath_leica-sp5-aobs.pdf Leica TCS SP5 AOBS] | ||
Revision as of 15:35, 23 May 2008
Confocal Microscopy
General Information
- Confocal Principle
The document shows a simplified beam path and the basic compounds of an inverted Confocal Laser Scanning Microscope (CLSM).
- Effect of the confocal pinhole
This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.
The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization.
