Technical Information
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* [http://web-redaktion.tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/teaching/microscopy/ibarra_how-to-set-koehler-illumination.pdf How to set Koehler illumination] | * [http://web-redaktion.tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/teaching/microscopy/ibarra_how-to-set-koehler-illumination.pdf How to set Koehler illumination] | ||
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== Confocal Microscopy == | == Confocal Microscopy == | ||
=== General Information === | === General Information === | ||
Revision as of 16:03, 23 May 2008
Contents |
Light Microscopy
Basics
Confocal Microscopy
General Information
- Confocal Principle
The document shows a simplified beam path and the basic compounds of an inverted Confocal Laser Scanning Microscope (CLSM).
- Effect of the confocal pinhole
This movie shows a volume representation of fluorescent beads, based on a confocal image stack. During the movie, the diameter of the confocal pinhole changes from fully open to 1 Airy Unit. One can see the contrast enhancement in X-Y direction as well as the change in the point spread function, once the pinhole is closed.
The beads were acquired with a Leica TCS SP5 and processed with Improvision Volocity Visualization.
Beam Paths
- Leica TCS SP5 AOBS
- Leica TCS SPE
- Leica TCS SP2 AOBS
- Nikon A1R
- Olympus FV1000
- Zeiss LSM 710
- Zeiss LSM 510 Meta
Laser Modules
- Zeiss LSM 510
- Leica TCS SP5
