W2 - DeltaVision Core
From BioDIP
(Difference between revisions)
Line 15: | Line 15: | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. | |microscope=manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. | ||
− | |obj1=Olympus | + | |obj1=Olympus UPlan SApochromat 10x 0.4 |
− | |obj2=Olympus | + | |obj2=Olympus UPlan SApochromat 20x 0.75 |
|obj3=Olympus UApochromat/340 40x 1.35 Oil | |obj3=Olympus UApochromat/340 40x 1.35 Oil | ||
− | |obj4=Olympus | + | |obj4=Olympus Plan ApochromatN 60x 1.42 Oil |
− | |obj5=Olympus | + | |obj5=Olympus UPlan SApochromat 100x 1.4 Oil |
− | |obj6=Olympus | + | |obj6=Olympus UApochromat 150x 1.45 Oil TIRFM |
− | |obj7=Olympus | + | |obj7=Olympus UPlan Apochromat 60x 1.20 Water |
+ | |obj8=Olympus UPlan SApochromat 100x 1.4 Oil | ||
|ill1=SSI-lumencor | |ill1=SSI-lumencor | ||
|ill2=Transmitted Light (LED) | |ill2=Transmitted Light (LED) |
Revision as of 10:40, 6 November 2014
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. | ||
objectives |
| ||
illumination | |||
detection | Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php | ||
reflectors | [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5
see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]] | ||
features | Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki. | ||
software | Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system. | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. | MOZGO 4076530000 10.1.145.25 cx63541s |