W2 - DeltaVision Core
From BioDIP
(Difference between revisions)
(removed from equipment list by "Not listed") |
|||
| (33 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
| − | |system-number=MPI | + | |system-number=MPI- W2 |
|system-name=DeltaVision Core | |system-name=DeltaVision Core | ||
| − | |type= | + | |type=Not listed |
|category=Wide-Field | |category=Wide-Field | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
| − | |room= | + | |room=U35 |
| + | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
| + | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. Motorised stage and environmental control. | |description=High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. Motorised stage and environmental control. | ||
|applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. | |applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. | ||
| − | |image= | + | |image=W2 - DeltaVision Core.jpg |
|stand-name=Olympus - IX71 | |stand-name=Olympus - IX71 | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=manual, with | + | |microscope=manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. |
| − | |obj1=Olympus | + | |obj1=Olympus UPlan SApochromat 10x 0.4 |
| − | |obj2=Olympus | + | |obj2=Olympus UPlan SApochromat 20x 0.75 |
| − | |obj3=Olympus | + | |obj3=Olympus UApochromat/340 40x 1.35 Oil |
| − | |obj4=Olympus | + | |obj4=Olympus Plan ApochromatN 60x 1.42 Oil |
| − | |obj5=Olympus | + | |obj5=Olympus UPlan SApochromat 100x 1.4 Oil |
| − | |obj6=Olympus | + | |obj6=Olympus UApochromat 150x 1.45 Oil TIRFM |
| − | |obj7=Olympus | + | |obj7=Olympus UPlan Apochromat 60x 1.20 Water |
| − | |ill1= | + | |obj8=Olympus UPlan SApochromat 100x 1.4 Oil |
| + | |ill1=SSI-lumencor | ||
|ill2=Transmitted Light (LED) | |ill2=Transmitted Light (LED) | ||
|detection=Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php | |detection=Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php | ||
| − | |reflectors= | + | |reflectors=Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5 |
| − | + | see [[File:Filter_Positions_Hard_Coat_10posEM-7posEP-DichroMs_2011.pdf]] | |
| − | + | ||
| − | Fast fluo emitter wheel in front of camera. | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
|features=Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples. | |features=Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples. | ||
| − | |software=Applied Precision SoftWorx running on a Cent OS Linux | + | |software=Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system. |
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
| − | |inv=MOZGO 4076530000 | + | |link0=[MPI-W2_-_DeltaVision_Core_-_Startup_%26_shutdown]] |
| + | |inv=MOZGO 4076530000 10.1.145.25 cx63541s | ||
}} | }} | ||
Latest revision as of 14:16, 3 February 2016
|
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Olympus - IX71, Inverted stand, manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. | ||
| objectives |
| ||
| illumination | |||
| detection | Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php | ||
| reflectors | [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5
see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]] | ||
| features | Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki. | ||
| software | Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system. | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | MOZGO 4076530000 10.1.145.25 cx63541s |
