W2 - DeltaVision Core
From BioDIP
(Difference between revisions)
Line 7: | Line 7: | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=036 | |room=036 | ||
+ | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
+ | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. Motorised stage and environmental control. | |description=High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. Motorised stage and environmental control. | ||
|applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. | |applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. |
Revision as of 12:26, 18 November 2013
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. | ||
objectives | |||
illumination | |||
detection | Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php | ||
reflectors | [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A584/Cy5
see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]] | ||
features | Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki. | ||
software | Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system. | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. | MOZGO 4076530000 10.1.145.25 cx63541 |