W2 - DeltaVision Core

Suitable for Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy..

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX71, Inverted stand, manual, with Applies Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control.
objectives	Olympus UPlanSApo 10x 0.4 Olympus UPlanSApo 20x 0.75 0.65mm WD Olympus UApo/340 40x 1.35 Oil Iris Olympus PlanApo N 60x 1.42 Oil UIS2 inf/0.17/FN26.5 WD 0.15 mm Olympus UPlanSApo 100x 1.4 Oil Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr Olympus UPlanApo 60x 1.20 Water PSF WD 0.25 mm
illumination	Fluorescence (Xenon) Transmitted Light (LED)
detection	Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php
reflectors	Modern hard coated "ET" filters (apart from DAPI exciter) Fast filter wheels for fluo exciters and ND filters in front of xenon lamp. D350/50x ET402/15x ET555/35x ET645/30x Fast fluo emitter wheel in front of camera. ET455/50m ET525/36m ET605/52m ET705/72m Manual emitters in front of eyepeices. DAPI, FTIC, TRITC. Polychromatic beam splitters for 1) DAPI, FITC, RD-TR-PE, Cy5 in filter cube position1: T89100bs. and 2) ET dualband GFP/mCherry in position 2. See spectra sheet at system.
features	Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki.
software	Applied Precision SoftWorx running on a Cent OS Linux box. Hardware controlled indirectly via a windows XP machine also on the system.
incubation	Cage incubator (T+CO2)
links	[ manual] [ laser power values] [ beam path] [ advanced system check]
inv.nr.	MOZGO 4076530000 10.1.145.25 cx63541

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