W2 - DeltaVision Core

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|obj6=Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr
 
|obj6=Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr
 
|obj7=Olympus UPlanApo 60x 1.20 Water PSF WD 0.25 mm
 
|obj7=Olympus UPlanApo 60x 1.20 Water PSF WD 0.25 mm
|ill1=Fluorescence (Xenon)
+
|ill1=SSI-lumencor
 
|ill2=Transmitted Light (LED)
 
|ill2=Transmitted Light (LED)
 
|detection=Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php
 
|detection=Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php
|reflectors=Modern hard coated "ET" filters (apart from DAPI exciter)
+
|reflectors=Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position  fluo emitter wheel in front of camera.
Fast filter wheels for fluo exciters and ND filters in front of xenon lamp.
+
see  [[File:Filter_Positions_Hard_Coat_10posEM-7posEP-DichroMs_2011.pdf]]
D350/50x ET402/15x ET555/35x ET645/30x
+
Fast fluo emitter wheel in front of camera.
+
ET455/50m ET525/36m ET605/52m ET705/72m
+
Manual emitters in front of eyepeices. DAPI, FTIC, TRITC.
+
Polychromatic beam splitters for 1) DAPI, FITC, RD-TR-PE, Cy5 in filter cube position1: T89100bs. and 2) ET dualband GFP/mCherry in position 2.
+
See spectra sheet at system.
+
 
|features=Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal.  Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples.
 
|features=Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal.  Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples.
 
|software=Applied Precision SoftWorx running on a Cent OS Linux box. Hardware controlled indirectly via a windows XP machine also on the system.
 
|software=Applied Precision SoftWorx running on a Cent OS Linux box. Hardware controlled indirectly via a windows XP machine also on the system.

Revision as of 16:52, 18 August 2011

Suitable for Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy..
MPI-W2.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX71, Inverted stand, manual, with Applies Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control.
objectives
Olympus UPlanSApo 10x 0.4
Olympus UPlanSApo 20x 0.75 0.65mm WD
Olympus UApo/340 40x 1.35 Oil Iris
Olympus PlanApo N 60x 1.42 Oil UIS2 inf/0.17/FN26.5 WD 0.15 mm
Olympus UPlanSApo 100x 1.4 Oil
Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr
Olympus UPlanApo 60x 1.20 Water PSF WD 0.25 mm
illumination
SSI-lumencor
Transmitted Light (LED)
detection Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php
reflectors [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera.

see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]]

features Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki.
software Applied Precision SoftWorx running on a Cent OS Linux box. Hardware controlled indirectly via a windows XP machine also on the system.
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZGO 4076530000 10.1.145.25 cx63541



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