W2 - DeltaVision Elite
From BioDIP
(Difference between revisions)
(creation of page; copy all information from old DV core page, still needs to be updated) |
|||
| Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
|system-number=MPI- W2 | |system-number=MPI- W2 | ||
| − | |system-name=DeltaVision | + | |system-name=DeltaVision Elite |
|type=Microscope | |type=Microscope | ||
|category=Wide-Field | |category=Wide-Field | ||
| Line 9: | Line 9: | ||
|Facility-logo=MPI-CBG-Lmf-logo-big.png | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
| − | |description=High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. | + | |description=High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. New generation motorised stage, Ultimate focus hardware autofocus system, and environmental control. |
| − | |applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. | + | |applications=Live cell imaging, time lapse, multi point, multi-well plates. Tile scanning large samples. Scanning multi-well plates; High resolution deconvolution fluorescence microscopy. |
|image=W2 - DeltaVision Core.jpg | |image=W2 - DeltaVision Core.jpg | ||
|stand-name=Olympus - IX71 | |stand-name=Olympus - IX71 | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=manual, with Applied Precision add on equipment: motorized stage, weather station black | + | |microscope=hybrid: partly motorized, partly manual, with Applied Precision add on equipment: new generation motorized stage, ULtimate focus; weather station black environmental control box, with custom built Co2 control. |
|obj1=Olympus UPlan SApochromat 10x 0.4 | |obj1=Olympus UPlan SApochromat 10x 0.4 | ||
|obj2=Olympus UPlan SApochromat 20x 0.75 | |obj2=Olympus UPlan SApochromat 20x 0.75 | ||
| Line 25: | Line 25: | ||
|ill1=SSI-lumencor | |ill1=SSI-lumencor | ||
|ill2=Transmitted Light (LED) | |ill2=Transmitted Light (LED) | ||
| − | |detection= | + | |detection=PCO sCMOS<br>Roper Evolve EMCCD |
|reflectors=Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5 | |reflectors=Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5 | ||
see [[File:Filter_Positions_Hard_Coat_10posEM-7posEP-DichroMs_2011.pdf]] | see [[File:Filter_Positions_Hard_Coat_10posEM-7posEP-DichroMs_2011.pdf]] | ||
Revision as of 16:53, 29 June 2015
|
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX71, Inverted stand, hybrid: partly motorized, partly manual, with Applied Precision add on equipment: new generation motorized stage, ULtimate focus; weather station black environmental control box, with custom built Co2 control. | ||
| objectives |
| ||
| illumination | |||
| detection | PCO sCMOS Roper Evolve EMCCD | ||
| reflectors | [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5
see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]] | ||
| features | Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki. | ||
| software | Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system. | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | MOZGO 4076530000 10.1.145.25 cx63541s |
