W4 - Zeiss Inverted CCD
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI-W4 | + | |system-number=MPI- W4 |
|system-name=Zeiss Inverted CCD | |system-name=Zeiss Inverted CCD | ||
|type=Microscope | |type=Microscope | ||
| Line 6: | Line 6: | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
| − | |room=microscopy 3rd floor north ( | + | |room=microscopy 3rd floor north (356) |
| − | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| + | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence | |description=Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence | ||
| − | |applications= | + | |applications=fixed samples, fluorescence, transmitted light, z stacks. |
| − | |image= | + | |image=W4 - Zeiss Inverted CCD.jpg |
| + | |stand-name=Zeiss - Axiovert 200M | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope= | + | |microscope=- |
| − | |obj1=Zeiss | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
| − | |obj2=Zeiss | + | |obj2=Zeiss Plan-Neofluar 40x 0.75 |
| − | |obj3=Zeiss LD Plan-Neofluar 20x 0.4 | + | |obj3=Zeiss Plan-Apochromat 20x 0.75 |
| − | | | + | |obj4=Zeiss Plan-Neofluar 63x 1.25 Oil |
| − | | | + | |obj5=Zeiss Plan-Neofluar 100x 1.3 Oil |
| + | |obj6=Zeiss Plan-Neofluar 5x 0.15 Ph1 | ||
| + | |obj7=Zeiss LD Plan-Neofluar 20x 0.4 Ph2 | ||
| + | |obj8=Zeiss Plan-Neofluar 1.25x 0.0035 | ||
| + | |obj9=Zeiss LD Achroplan 40x 0.6 Ph2 | ||
| + | |obj10=Zeiss Plan-Neofluar 40x 1.3 Oil Ph3 | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
| − | |detection=Diagnostic Instruments RT Spot | + | |detection=Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080, |
| + | 1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm | ||
|reflectors=DAPI (X BP 361-396, D BP 422-639, M BP 434-486)<br>eGFP (X BP 449-488, D BP 495-598, M BP 501-551)<br>TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)<br>Cy5 (X BP 604-649, D LP 664, M LP 661)<br>DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)<br> | |reflectors=DAPI (X BP 361-396, D BP 422-639, M BP 434-486)<br>eGFP (X BP 449-488, D BP 495-598, M BP 501-551)<br>TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)<br>Cy5 (X BP 604-649, D LP 664, M LP 661)<br>DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)<br> | ||
|features=- | |features=- | ||
| + | |software=- | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
| − | |inv=402166 | + | |inv=402166 10.1.30.236 |
| + | |facility_image=logo-lmf.jpg | ||
|objectives=Plan Neofluar 5x/0.15 Ph1<br>Plan Neofluar 10x/0.25 Ph1<br>LD Plan Neofluar 20x/0.40 Korr Ph2<br>LD Plan Neofluar 40x/0.60 Korr Ph2<br>Plan Apochromat 100x/1.4 Oil DIC<br> | |objectives=Plan Neofluar 5x/0.15 Ph1<br>Plan Neofluar 10x/0.25 Ph1<br>LD Plan Neofluar 20x/0.40 Korr Ph2<br>LD Plan Neofluar 40x/0.60 Korr Ph2<br>Plan Apochromat 100x/1.4 Oil DIC<br> | ||
|illumination=Halogen<br>HBO | |illumination=Halogen<br>HBO | ||
}} | }} | ||
Latest revision as of 18:27, 24 November 2014
|
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Zeiss - Axiovert 200M, Inverted stand, - | ||
| objectives |
| ||
| illumination | |||
| detection | Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080,
1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm | ||
| reflectors | DAPI (X BP 361-396, D BP 422-639, M BP 434-486) eGFP (X BP 449-488, D BP 495-598, M BP 501-551) TexasRed ( X BP 540-580, D LP 588 , M BP 595-667) Cy5 (X BP 604-649, D LP 664, M LP 661) DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III) | ||
| features | - | ||
| software | - | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. | 402166 10.1.30.236 |
