W4 - Zeiss Inverted CCD

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|building=MPI-CBG
 
|building=MPI-CBG
 
|room=microscopy 3rd floor north (356)
 
|room=microscopy 3rd floor north (356)
 +
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 +
|Institute-logo=Logo MPI-CBG.jpg
 
|description=Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence
 
|description=Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence
 
|applications=fixed samples, fluorescence, transmitted light, z stacks.
 
|applications=fixed samples, fluorescence, transmitted light, z stacks.
|image=MPI10.jpg
+
|image=W4 - Zeiss Inverted CCD.jpg
 
|stand-name=Zeiss - Axiovert 200M
 
|stand-name=Zeiss - Axiovert 200M
 
|stand=Inverted
 
|stand=Inverted
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|obj5=Zeiss Plan-Neofluar 100x 1.3 Oil
 
|obj5=Zeiss Plan-Neofluar 100x 1.3 Oil
 
|obj6=Zeiss Plan-Neofluar 5x 0.15 Ph1
 
|obj6=Zeiss Plan-Neofluar 5x 0.15 Ph1
|obj7=Zeiss LC Plan-Neofluar 20x 0.4
+
|obj7=Zeiss LD Plan-Neofluar 20x 0.4 Ph2
 +
|obj8=Zeiss Plan-Neofluar 1.25x 0.0035
 +
|obj9=Zeiss LD Achroplan 40x 0.6 Ph2
 +
|obj10=Zeiss Plan-Neofluar 40x 1.3 Oil Ph3
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (HBO)
 
|ill2=Fluorescence (HBO)

Latest revision as of 18:27, 24 November 2014

Suitable for fixed samples, fluorescence, transmitted light, z stacks..
W4 - Zeiss Inverted CCD.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, microscopy 3rd floor north (356))

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Neofluar 40x 0.75
Zeiss Plan-Apochromat 20x 0.75
Zeiss Plan-Neofluar 63x 1.25 Oil
Zeiss Plan-Neofluar 100x 1.3 Oil
Zeiss Plan-Neofluar 5x 0.15 Ph1
Zeiss LD Plan-Neofluar 20x 0.4 Ph2
Zeiss Plan-Neofluar 1.25x 0.0035
Zeiss LD Achroplan 40x 0.6 Ph2
Zeiss Plan-Neofluar 40x 1.3 Oil Ph3
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
detection Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080,

1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm

reflectors DAPI (X BP 361-396, D BP 422-639, M BP 434-486)
eGFP (X BP 449-488, D BP 495-598, M BP 501-551)
TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)
Cy5 (X BP 604-649, D LP 664, M LP 661)
DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)
features -
software -
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 402166 10.1.30.236



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