W4 - Zeiss Inverted CCD

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|inv=402166 10.1.30.236
 
|facility_image=logo-lmf.jpg
 
|facility_image=logo-lmf.jpg
 
|objectives=Plan Neofluar 5x/0.15 Ph1<br>Plan Neofluar 10x/0.25 Ph1<br>LD Plan Neofluar 20x/0.40 Korr Ph2<br>LD Plan Neofluar 40x/0.60 Korr Ph2<br>Plan Apochromat 100x/1.4 Oil DIC<br>
 
|objectives=Plan Neofluar 5x/0.15 Ph1<br>Plan Neofluar 10x/0.25 Ph1<br>LD Plan Neofluar 20x/0.40 Korr Ph2<br>LD Plan Neofluar 40x/0.60 Korr Ph2<br>Plan Apochromat 100x/1.4 Oil DIC<br>
 
|illumination=Halogen<br>HBO
 
|illumination=Halogen<br>HBO
 
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Revision as of 17:55, 8 September 2011

Suitable for fixed samples, fluorescence, transmitted light, z stacks..
MPI10.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, microscopy 3rd floor north (356))

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axiovert 200M, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Neofluar 40x 0.75
Zeiss Plan-Apochromat 20x 0.75
Zeiss Plan-Neofluar 63x 1.25 Oil
Zeiss Plan-Neofluar 100x 1.3 Oil
Zeiss Plan-Neofluar 5x 0.15 Ph1
Zeiss LC Plan-Neofluar 20x 0.4
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
detection Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080,

1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm

reflectors DAPI (X BP 361-396, D BP 422-639, M BP 434-486)
eGFP (X BP 449-488, D BP 495-598, M BP 501-551)
TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)
Cy5 (X BP 604-649, D LP 664, M LP 661)
DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)
features -
software -
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 402166 10.1.30.236



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