W4 - Zeiss Inverted CCD

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Suitable for fixed samples, fluorescence, transmitted light, z stacks..
MPI10.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, microscopy 3rd floor north (353))

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axiovert 200M, Inverted stand, -
objectives
Zeiss EC Plan-Neofluar 5x 0.16
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss LD Plan-Neofluar 20x 0.4
Zeiss EC Plan-Neofluar 40x 0.75
Zeiss Plan-Apochromat 100x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
detection Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080,

1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm

reflectors DAPI (X BP 361-396, D BP 422-639, M BP 434-486)
eGFP (X BP 449-488, D BP 495-598, M BP 501-551)
TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)
Cy5 (X BP 604-649, D LP 664, M LP 661)
DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)
features -
software -
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 402166



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