W4 - Zeiss Inverted CCD
From BioDIP
(Difference between revisions)
| Line 8: | Line 8: | ||
|room=microscopy 3rd floor north (353) | |room=microscopy 3rd floor north (353) | ||
|description=Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence | |description=Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence | ||
| − | |applications= | + | |applications=fixed samples, fluorescence, transmitted light, z stacks. |
|image=MPI10.jpg | |image=MPI10.jpg | ||
|stand-name=Zeiss - Axiovert 200M | |stand-name=Zeiss - Axiovert 200M | ||
| Line 20: | Line 20: | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
| − | |detection=Diagnostic Instruments RT Spot | + | |detection=Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080, |
| + | 1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm | ||
|reflectors=DAPI (X BP 361-396, D BP 422-639, M BP 434-486)<br>eGFP (X BP 449-488, D BP 495-598, M BP 501-551)<br>TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)<br>Cy5 (X BP 604-649, D LP 664, M LP 661)<br>DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)<br> | |reflectors=DAPI (X BP 361-396, D BP 422-639, M BP 434-486)<br>eGFP (X BP 449-488, D BP 495-598, M BP 501-551)<br>TexasRed ( X BP 540-580, D LP 588 , M BP 595-667)<br>Cy5 (X BP 604-649, D LP 664, M LP 661)<br>DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III)<br> | ||
|features=- | |features=- | ||
Revision as of 16:41, 27 July 2011
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Zeiss - Axiovert 200M, Inverted stand, - | ||
| objectives | |||
| illumination | |||
| detection | Diagnostic Instruments RT Spot. Camera pixel spacing approx. 7.4 microns. Ruler measurements give 40x lens at 0.182 microns per pixel, 63x lens at 0.166 microns per pixel. 1520 x 1080,
1,641,600 pixels per image, binning 2,3,4x. see more in fo here: http://www.benderassoc.com/mic/digital&analog%20imaging/DVC/DiagnosticsCameras/spotrtsp.htm | ||
| reflectors | DAPI (X BP 361-396, D BP 422-639, M BP 434-486) eGFP (X BP 449-488, D BP 495-598, M BP 501-551) TexasRed ( X BP 540-580, D LP 588 , M BP 595-667) Cy5 (X BP 604-649, D LP 664, M LP 661) DIC (BF, Ph1, Ph2, Ph3, DIC II, and DIC III) | ||
| features | - | ||
| software | - | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. | 402166 |
