W7 - Zeiss Laser Microdissection

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{{Equipment
 
{{Equipment
|system-number=
+
|system-number=MPI- W7
|system-name=
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|system-name=Zeiss Laser Microdissection
|type=
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|type=Microscope
|category=
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|category=Dissection
|facility=
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|facility=MPI-CBG LMF
|building=
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|building=MPI-CBG
|room=
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|room=U36
|Facility-logo=
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|Facility-logo=MPI-CBG-Lmf-logo-big.png
|Institute-logo=
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|Institute-logo=Logo MPI-CBG.jpg
|description=
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|description=a laser microdissection system based on an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color)
|applications=
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|applications=the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system.
|image=
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|image=W7 - Zeiss Laser Microdissection new.jpg
|stand-name=
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|stand-name=Zeiss - Axio Observer.Z1
|stand=
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|stand=Inverted
|microscope=
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|obj1=Zeiss EC Plan Neofluar 2.5x 0.085
|obj1=
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|obj2=Zeiss Fluar 5x 0.25
|obj2=
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|obj3=Zeiss Fluar 10x 0.5
|obj3=
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|obj4=Zeiss LD Plan-Neofluar 20x 0.4
|obj4=
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|obj5=Zeiss LD Plan-Neofluar 40x 0.6
|obj5=
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|obj6=Zeiss LD Plan Neofluar 63x 0.75
|obj6=
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|obj7=Zeiss EC Plan-Neofluar 100x 1.3 Oil
|obj7=
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|obj8=Zeiss Plan-Apochromat 150x 1.35 Glycerine
|obj8=
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|ill1=Laser Diode 355 nm
|obj9=
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|ill2=Colibri LED 365+470+590+625nm
|obj10=
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|ill3=Fluorescence (Metal Halide, HXP, 120W)
|ill1=
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|ill4=Transmitted Light (LED)
|ill2=
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|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w <br>
|ill3=
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AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area)
|ill4=
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|reflectors=Pos 1: 100% mirror TL (for UV-cutting) <br>
|ill5=
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Pos 2: FS09 AF 488 RL <br>
|ill6=
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Pos 3: FS14 AF 546 RL <br>
|ill7=
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Pos 4: FS34 BFP RL <br>
|ill8=
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Pos 5: FS44 FITC RL <br>
|ill9=
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Pos 6: FS62 HE B/G/HR RL <br>
|ill10=
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|features=Robo Microbeam UV laser microdissection system
|detection=
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|software=Axiovision  + PalmRobo 4.6
|reflectors=
+
|incubation=Not available
|features=
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|inv=MOZGO 4093910000; Zeiss SIP 1024672713
|software=
+
|incubation=
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|link0=
+
|link1=
+
|link2=
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|link3=
+
|inv=
+
 
}}
 
}}

Latest revision as of 17:54, 3 February 2016

Suitable for the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system."the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system." cannot be used as a page name in this wiki..
W7 - Zeiss Laser Microdissection new.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U36)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand,
objectives
Zeiss EC Plan Neofluar 2.5x 0.085
Zeiss Fluar 5x 0.25
Zeiss Fluar 10x 0.5
Zeiss LD Plan-Neofluar 20x 0.4
Zeiss LD Plan-Neofluar 40x 0.6
Zeiss LD Plan Neofluar 63x 0.75
Zeiss EC Plan-Neofluar 100x 1.3 Oil
Zeiss Plan-Apochromat 150x 1.35 Glycerine
illumination
Laser Diode 355 nm
Colibri LED 365+470+590+625nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (LED)
detection Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w

AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area)

reflectors Pos 1: 100% mirror TL (for UV-cutting)

Pos 2: FS09 AF 488 RL
Pos 3: FS14 AF 546 RL
Pos 4: FS34 BFP RL
Pos 5: FS44 FITC RL
Pos 6: FS62 HE B/G/HR RL

features Robo Microbeam UV laser microdissection system
software Axiovision + PalmRobo 4.6
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZGO 4093910000; Zeiss SIP 1024672713



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