W7 - Zeiss Laser Microdissection
From BioDIP
(Difference between revisions)
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|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
− | |room= | + | |room=U36 |
|Facility-logo=MPI-CBG-Lmf-logo-big.png | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
− | |description= | + | |description=a laser microdissection system based on an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color) |
− | |applications= | + | |applications=the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system. |
|image=W7 - Zeiss Laser Microdissection.JPG | |image=W7 - Zeiss Laser Microdissection.JPG | ||
− | |stand-name= | + | |stand-name=Zeiss - Axio Observer.Z1 |
− | |stand= | + | |stand=Inverted |
− | |incubation= | + | |obj1=Zeiss EC Plan Neofluar 2.5x 0.075 |
+ | |obj2=Zeiss Fluar 5x 0.25 | ||
+ | |obj3=Zeiss Fluar 10x 0.5 | ||
+ | |obj4=Zeiss LD Plan-Neofluar 20x 0.4 | ||
+ | |obj5=Zeiss LD Plan-Neofluar 40x 0.6 | ||
+ | |obj6=Zeiss LD-Achroplan 63x 0.75 Ph2 | ||
+ | |obj7=Zeiss EC Plan-Neofluar 100x 1.3 Oil | ||
+ | |obj8=Zeiss Plan-Apochromat 150x 1.35 Glycerine | ||
+ | |ill1=Laser Diode 355 nm | ||
+ | |ill2=Colibri LED 365+470+590+625nm | ||
+ | |ill3=Fluorescence (Metal Halide, HXP, 120W) | ||
+ | |ill4=Transmitted Light (LED) | ||
+ | |detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w <br> | ||
+ | AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) | ||
+ | |reflectors=DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 <br> | ||
+ | GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br> | ||
+ | Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br> | ||
+ | Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50 <br> | ||
+ | GFP + UV-Cut: EX 470/40 ; BS 510 ; EM LP 515 | ||
+ | |software=Axiovision + PalmRobo 4.6 | ||
+ | |incubation=Not available | ||
+ | |inv=MOZGO 4093910000; Zeiss SIP 1024672713 | ||
}} | }} |
Revision as of 17:41, 1 June 2015
250px |
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Zeiss - Axio Observer.Z1, Inverted stand, | ||
objectives | |||
illumination | |||
detection | Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) | ||
reflectors | DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 | ||
features | |||
software | Axiovision + PalmRobo 4.6 | ||
incubation | Not available | ||
links |
| ||
inv.nr. | MOZGO 4093910000; Zeiss SIP 1024672713 |