W7 - Zeiss Laser Microdissection

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|stand-name=Zeiss - Axio Observer.Z1
 
|stand-name=Zeiss - Axio Observer.Z1
 
|stand=Inverted
 
|stand=Inverted
−
|obj1=Zeiss EC Plan Neofluar 2.5x 0.075
+
|obj1=Zeiss EC Plan Neofluar 2.5x 0.085
 
|obj2=Zeiss Fluar 5x 0.25
 
|obj2=Zeiss Fluar 5x 0.25
 
|obj3=Zeiss Fluar 10x 0.5
 
|obj3=Zeiss Fluar 10x 0.5
 
|obj4=Zeiss LD Plan-Neofluar 20x 0.4
 
|obj4=Zeiss LD Plan-Neofluar 20x 0.4
 
|obj5=Zeiss LD Plan-Neofluar 40x 0.6
 
|obj5=Zeiss LD Plan-Neofluar 40x 0.6
−
|obj6=Zeiss LD-Achroplan 63x 0.75 Ph2
+
|obj6=Zeiss LD Plan Neofluar 63x 0.75
 
|obj7=Zeiss EC Plan-Neofluar 100x 1.3 Oil
 
|obj7=Zeiss EC Plan-Neofluar 100x 1.3 Oil
 
|obj8=Zeiss Plan-Apochromat 150x 1.35 Glycerine
 
|obj8=Zeiss Plan-Apochromat 150x 1.35 Glycerine

Revision as of 17:46, 1 June 2015

Suitable for the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system."the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system." cannot be used as a page name in this wiki..
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Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U36)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Observer.Z1, Inverted stand,
objectives
Zeiss EC Plan Neofluar 2.5x 0.085
Zeiss Fluar 5x 0.25
Zeiss Fluar 10x 0.5
Zeiss LD Plan-Neofluar 20x 0.4
Zeiss LD Plan-Neofluar 40x 0.6
Zeiss LD Plan Neofluar 63x 0.75
Zeiss EC Plan-Neofluar 100x 1.3 Oil
Zeiss Plan-Apochromat 150x 1.35 Glycerine
illumination
Laser Diode 355 nm
Colibri LED 365+470+590+625nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (LED)
detection Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w

AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area)

reflectors DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60

GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50
Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70
Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50
GFP + UV-Cut: EX 470/40 ; BS 510 ; EM LP 515

features
software Axiovision + PalmRobo 4.6
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZGO 4093910000; Zeiss SIP 1024672713


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