WF Laser Microdissection, CRTD
From BioDIP
(Difference between revisions)
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{{Equipment | {{Equipment | ||
− | |system-number= | + | |system-number=BIO19 |
|system-name=PALM, inverse, CRTD | |system-name=PALM, inverse, CRTD | ||
|type=Microscope | |type=Microscope | ||
− | |category= | + | |category=Dissection |
− | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
|building=CRTD | |building=CRTD | ||
|room=-1.121 | |room=-1.121 | ||
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
− | |description= | + | |description=it is a laser microdissection system consisting of an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color) |
− | |applications= | + | |applications=the manipulation of biological samples with a UV laser and the collection of cut material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working imaging system. |
+ | |image=PALM, inverse, CRTD.jpg | ||
|stand-name=AxioObserver.Z1 | |stand-name=AxioObserver.Z1 | ||
|stand=inverted | |stand=inverted | ||
− | |||
− | |||
|obj2=Zeiss Fluar 5x 0.25 | |obj2=Zeiss Fluar 5x 0.25 | ||
− | |obj3=Zeiss LD Plan-Neofluar 20x 0.4 | + | |obj3=Zeiss LD Plan-Neofluar 20x 0.4 Ph2 |
|obj4=Zeiss LD Plan-Neofluar 40x 0.6 | |obj4=Zeiss LD Plan-Neofluar 40x 0.6 | ||
|obj5=Zeiss Plan-Apochromat 150x 1.35 Glycerine | |obj5=Zeiss Plan-Apochromat 150x 1.35 Glycerine | ||
+ | |obj6=Zeiss EC Plan-Neofluar 40x 1.3 Oil | ||
+ | |obj7=Zeiss EC Plan-Neofluar 10x 0.3 | ||
|ill1=Laser Diode 355 nm | |ill1=Laser Diode 355 nm | ||
|ill2=Fluorescence (Metal Halide, HXP, 120W) | |ill2=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill3=Transmitted Light (Halogen) | |ill3=Transmitted Light (Halogen) | ||
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w <br> | |detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w <br> | ||
− | AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) | + | AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) |
|reflectors=DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 <br> | |reflectors=DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 <br> | ||
GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br> | GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br> | ||
Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br> | Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br> | ||
− | Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50 | + | Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50 <br> |
+ | GFP (no cutting): EX 470/40 ; BS 510 ; EM LP 515 | ||
|features=- | |features=- | ||
|software=AxioVision | |software=AxioVision | ||
|incubation=Not available | |incubation=Not available | ||
− | |inv= | + | |inv=908140 |
}} | }} |
Latest revision as of 16:00, 15 February 2017
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
microscope | AxioObserver.Z1, inverted stand, | ||
objectives | |||
illumination | |||
detection | Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) | ||
reflectors | DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 | ||
features | - | ||
software | AxioVision | ||
incubation | Not available | ||
links |
| ||
inv.nr. | 908140 |