WF Laser Microdissection, CRTD

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|system-name=PALM, inverse, CRTD
 
|system-name=PALM, inverse, CRTD
 
|type=Microscope
 
|type=Microscope
|category=Laser Microdissection
+
|category=Dissection
 
|facility=Imaging@BIOTEC/CRTD
 
|facility=Imaging@BIOTEC/CRTD
 
|building=CRTD
 
|building=CRTD
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|stand-name=AxioObserver.Z1
 
|stand-name=AxioObserver.Z1
 
|stand=inverted
 
|stand=inverted
|obj1=Zeiss EC Plan Neofluar 2.5x 0.075
 
 
|obj2=Zeiss Fluar 5x 0.25
 
|obj2=Zeiss Fluar 5x 0.25
|obj3=Zeiss LD Plan-Neofluar 20x 0.4
+
|obj3=Zeiss LD Plan-Neofluar 20x 0.4 Ph2
 
|obj4=Zeiss LD Plan-Neofluar 40x 0.6
 
|obj4=Zeiss LD Plan-Neofluar 40x 0.6
 
|obj5=Zeiss Plan-Apochromat 150x 1.35 Glycerine
 
|obj5=Zeiss Plan-Apochromat 150x 1.35 Glycerine
 +
|obj6=Zeiss EC Plan-Neofluar 40x 1.3 Oil
 +
|obj7=Zeiss EC Plan-Neofluar 10x 0.3
 
|ill1=Laser Diode 355 nm
 
|ill1=Laser Diode 355 nm
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
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GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br>
 
GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br>
 
Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br>
 
Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br>
Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50
+
Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50 <br>
 +
GFP (no cutting): EX 470/40 ; BS 510 ; EM LP 515
 
|features=-
 
|features=-
 
|software=AxioVision
 
|software=AxioVision
 
|incubation=Not available
 
|incubation=Not available
|inv=-
+
|inv=908140
 
}}
 
}}

Latest revision as of 16:00, 15 February 2017

Suitable for the manipulation of biological samples with a UV laser and the collection of cut material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working imaging system..
PALM, inverse, CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.121)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope AxioObserver.Z1, inverted stand,
objectives
Zeiss Fluar 5x 0.25
Zeiss LD Plan-Neofluar 20x 0.4 Ph2
Zeiss LD Plan-Neofluar 40x 0.6
Zeiss Plan-Apochromat 150x 1.35 Glycerine
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss EC Plan-Neofluar 10x 0.3
illumination
Laser Diode 355 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w

AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area)

reflectors DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60

GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50
Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70
Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50
GFP (no cutting): EX 470/40 ; BS 510 ; EM LP 515

features -
software AxioVision
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 908140



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