WF Laser Microdissection, CRTD
From BioDIP
(Difference between revisions)
(Created page with "{{Equipment |system-number=BIO17 |system-name=PALM, inverse, CRTD |type=Microscope |category=Laser Microdissection |facility=Imaging@Biotec |building=CRTD |room=-1.121 |Facili...") |
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|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
| − | |description= | + | |description=it is a laser microdissection system consisting of an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color) |
| − | |applications= | + | |applications=the manipulation of biological samples with a UV laser and the collection of cut material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working imaging system. |
| + | |||
|stand-name=AxioObserver.Z1 | |stand-name=AxioObserver.Z1 | ||
|stand=inverted | |stand=inverted | ||
| − | |||
|obj1=Zeiss EC Plan Neofluar 2.5x 0.075 | |obj1=Zeiss EC Plan Neofluar 2.5x 0.075 | ||
|obj2=Zeiss Fluar 5x 0.25 | |obj2=Zeiss Fluar 5x 0.25 | ||
| Line 23: | Line 23: | ||
|ill3=Transmitted Light (Halogen) | |ill3=Transmitted Light (Halogen) | ||
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w <br> | |detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w <br> | ||
| − | AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) | + | AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) |
|reflectors=DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 <br> | |reflectors=DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 <br> | ||
GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br> | GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 <br> | ||
Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br> | Cy3 + UV-Cut: EX 524/25 ; BS 561 ; EM 605/70 <br> | ||
| − | Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50 | + | Cy5 + UV-Cut: EX 640/30 ; BS 635 ; EM 690/50 |
|features=- | |features=- | ||
|software=AxioVision | |software=AxioVision | ||
Revision as of 15:20, 8 March 2013
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | AxioObserver.Z1, inverted stand, | ||
| objectives | |||
| illumination | |||
| detection | Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w AxioCam ICc with b/w and color option (1388 x 1038 pixel; 4,65x4,65 μm; 16,3x4,8 mm active area) | ||
| reflectors | DAPI + UV-Cut: EX 367/11 ; BS 405 ; EM 447/60 GFP + UV-Cut: EX 470/40 ; BS 488 ; EM 525/50 | ||
| features | - | ||
| software | AxioVision | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | - |
