WF Leica inverse, CRTD

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|type=Microscope
 
|type=Microscope
 
|category=Wide-Field
 
|category=Wide-Field
|facility=Imaging@Biotec
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|facility=Imaging@BIOTEC/CRTD
 
|building=CRTD
 
|building=CRTD
|room=-1.123
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|room=0
 
|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
|description=Our inverted Leica microscope is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to color co-site sampling function of the camera.
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|description=The inverted Leica microscope is designed for routine work on fixed fluorescent sample.
|applications=-
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|applications=The instrument is especially suitable for multicolor fluorescent samples due to high sensitivity b/w camera.
 
|image=WF Leica inverse, CRTD.jpg
 
|image=WF Leica inverse, CRTD.jpg
 
|stand-name=Leica - DMI4000
 
|stand-name=Leica - DMI4000
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=inverted stand, manual XYZ stage, fluorescence, transmitted light with automatic DIC, color and b/w acquisition
 
|microscope=inverted stand, manual XYZ stage, fluorescence, transmitted light with automatic DIC, color and b/w acquisition
|obj1=Leica HCX PL FLUOTAR 5x 0.15
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|obj1=Leica HCX PL S-APO 10x 0.3
|obj2=Leica HCX PL S-APO 10x 0.3
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|obj2=Leica HCX PL Fluotar L 20x 0.4
|obj3=Leica HCX PL Fluotar L 20x 0.4
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|obj3=Leica HCX PL FL L 40x 0.60
|obj4=Leica HCX PL FL L 40x 0.60
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|obj4=Leica HCX PL FLUOTAR 5x 0.15
|obj5=Leica HCX PL APO 63x 1.2 W
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|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
|detection=CCD Leica DFC 500 (1360*1024 up to 4080*3072, 6,45*6,45µm pixel)
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|detection=CCD Leica DFC 350 fx (1392*1040, 6,45*6,45µm pixel)
 
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>
 
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>
FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>
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GFP : EX 470/40 ; BS 495 ; EM 525/50<br>
TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590<br>
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Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>
CY5 : EX 628/40 ; BS 660 ; EM BP 692/40
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CFP : EX 436/20 ; BS 455 ; EM 480/40<br>
 +
YFP : EX 490/20 ; BS 515 ; EM 535/30<br>
 +
TL/DIC Analysator
 
|features=sequential imaging, Brightfield and Fluorescence overlay
 
|features=sequential imaging, Brightfield and Fluorescence overlay
|software=LAS AF
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|software=LAS
 
|incubation=Not available
 
|incubation=Not available
 
|link1=-
 
|link1=-

Latest revision as of 10:37, 13 February 2017

Suitable for The instrument is especially suitable for multicolor fluorescent samples due to high sensitivity b/w camera..
WF Leica inverse, CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, 0)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Leica - DMI4000, Inverted stand, inverted stand, manual XYZ stage, fluorescence, transmitted light with automatic DIC, color and b/w acquisition
objectives
Leica HCX PL S-APO 10x 0.3
Leica HCX PL Fluotar L 20x 0.4
Leica HCX PL FL L 40x 0.60
Leica HCX PL FLUOTAR 5x 0.15
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection CCD Leica DFC 350 fx (1392*1040, 6,45*6,45µm pixel)
reflectors DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425

GFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3 : EX 545/25 ; BS 565 ; EM 605/70
CFP : EX 436/20 ; BS 455 ; EM 480/40
YFP : EX 490/20 ; BS 515 ; EM 535/30
TL/DIC Analysator

features sequential imaging, Brightfield and Fluorescence overlay
software LAS
incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 72003



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