WF Leica inverse, CRTD

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|stand=Inverted
 
|stand=Inverted
 
|microscope=inverted stand, manual XYZ stage, fluorescence, transmitted light with automatic DIC, color and b/w acquisition
 
|microscope=inverted stand, manual XYZ stage, fluorescence, transmitted light with automatic DIC, color and b/w acquisition
|obj1=Leica HCX PL FLUOTAR 5x 0.15
+
|obj1=Leica HCX PL S-APO 10x 0.3
|obj2=Leica HCX PL S-APO 10x 0.3
+
|obj2=Leica HCX PL Fluotar L 20x 0.4
|obj3=Leica HCX PL Fluotar L 20x 0.4
+
|obj3=Leica HCX PL FL L 40x 0.60
|obj4=Leica HCX PL FL L 40x 0.60
+
|obj5=Leica HCX PL APO 63x 1.2 W
+
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
 
|detection=CCD Leica DFC 500 (1360*1024 up to 4080*3072, 6,45*6,45µm pixel)
 
|detection=CCD Leica DFC 500 (1360*1024 up to 4080*3072, 6,45*6,45µm pixel)
 
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>
 
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>
FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>
+
GFP : EX 470/40 ; BS 495 ; EM 525/50<br>
TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590<br>
+
Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>
CY5 : EX 628/40 ; BS 660 ; EM BP 692/40
+
CFP : EX 436/20 ; BS 455 ; EM BP 480/40<br>
 +
YFP : EX 490/20 ; BS 155 ; EM BP 535/30<br>
 +
TL/DIC Analysator
 
|features=sequential imaging, Brightfield and Fluorescence overlay
 
|features=sequential imaging, Brightfield and Fluorescence overlay
|software=LAS  
+
|software=LAS
 
|incubation=Not available
 
|incubation=Not available
 
|link1=-
 
|link1=-

Revision as of 15:45, 26 June 2014

Suitable for -.
WF Leica inverse, CRTD.jpg


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 220)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Leica - DMI4000, Inverted stand, inverted stand, manual XYZ stage, fluorescence, transmitted light with automatic DIC, color and b/w acquisition
objectives
Leica HCX PL S-APO 10x 0.3
Leica HCX PL Fluotar L 20x 0.4
Leica HCX PL FL L 40x 0.60
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection CCD Leica DFC 500 (1360*1024 up to 4080*3072, 6,45*6,45µm pixel)
reflectors DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425

GFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3 : EX 545/25 ; BS 565 ; EM 605/70
CFP : EX 436/20 ; BS 455 ; EM BP 480/40
YFP : EX 490/20 ; BS 155 ; EM BP 535/30
TL/DIC Analysator

features sequential imaging, Brightfield and Fluorescence overlay
software LAS
incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 72003



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