WF Olympus upright, BIOTEC

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{{Equipment
 
{{Equipment
| system-number       = 12
+
|system-number=BIO22
| system-name         = Olympus Videomicroscope upright
+
|system-name=WF Olympus upright, BIOTEC
| category             = Wide-Field
+
|type=Microscope
| stand                = upright
+
|category=Wide-Field
| image                = Bioz12.jpg
+
|facility=Imaging@BIOTEC/CRTD
| facility            = Imaging@Biotec
+
|building=BIOTEC
| facility_image      = logo-imagingatbiotec.jpg
+
|room=227
| building            = Biotec
+
|Facility-logo=Imaging@Biotec.jpg
| room                = 229
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|Institute-logo=Biotec logo.png
| description         = Our Olympus videomicroscope upright is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to the use of two possible cameras, a b/w Photometrics and a color Spot CCD. Timelapse, along with z-stacks can be performed with the use of motorized z-stage
+
|description=Our Olympus videomicroscope upright is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to the use of two possible cameras, a b/w Photometrics and a color Spot CCD. Timelapse, along with z-stacks can be performed with the use of motorized z-stage
| microscope          = upright stand, manual XY stage, motorized Z-stage, fluorescence, transmitted light with manual DIC, color and b/w acquisition
+
|applications=-
| objectives          = 4x/0.13<br>10x/0.3 Ph1<br>20x/0.5 Ph1 DIC<br>40x/0.75 Ph2 DIC<br>100x/1.35-0.5 Oil Ph3 DIC
+
|image=Bioz12.jpg
| illumination        = HBO lamp<br>Halogen lamp
+
|stand-name=Olympus - BX 61
| detection           = CCD Leica DFC 500 (1360*1024 up to 4080*3072, 6,45*6,45µm pixel)
+
|stand=Upright
| reflectors           = DAPI : EX 365/10 ; BS 400 ; EM LP 420<br>CFP : EX 436/20 ; BS 455 ; EM BP 480/40<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>Cy5 : EX 620/60 ; BS 660 ; EM 700/75
+
|microscope=manual XY stage, motorized Z-stage, fluorescence, transmitted light with manual DIC, color and b/w acquisition
| features             = timelapse imaging, z-stack, Brightfield, Fluorescence
+
|obj1=Olympus UPlanFl 4x 0.13
| incubation           = None
+
|obj2=Olympus UPlanSApo 10x 0.4
 +
|obj3=Olympus UPlanFl 20x 0.5 Ph1
 +
|obj4=Olympus UPlanSApo 20x 0.85 Oil
 +
|obj5=Olympus UPlanFLN 40X 0.75 Ph2
 +
|obj6=Olympus UPlanSApo 60x 1.35 Oil
 +
|obj7=Olympus UPlanApo 100x 1.35 oil Ph3
 +
|obj8=Olympus UPlanSApo 40x 0.95
 +
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (HBO)
 +
|detection=Coolsnap fx (1300x1030 pixel; 6,7x6,7μm; 12bit@20MHz) <br>
 +
Spot Insight QE Color (2048x2048 pixel; 7,4x7,4μm; 14bit@20MHz) <br>
 +
Olympus DP71 color (1360x1024, up to 4080x3072 by pixel shift; 6,45x6x45 µm)
 +
|reflectors=DAPI : EX 365/10 ; BS 400 ; EM LP 420<br>
 +
GFP/FITC : EX 480/20 ; BS 495 ; EM 525/50<br>
 +
GFP/FITC : EX 470/40 ; BS 495 ; EM 525/50<br>
 +
Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>
 +
Cy5 : EX 620/60 ; BS 660 ; EM 700/75<br>
 +
TRITC : EX 540/20 ; BS 570 ; EM 590
 +
|features=timelapse imaging, z-stack, Brightfield, Fluorescence
 +
|software=MetaMorph
 +
SPOT Basic/Advanced
 +
|incubation=Not available
 +
|link1=-
 +
|link2=-
 +
|inv=106553
 +
|facility_image=Imaging@Biotec.jpg
 +
|logo_image=Logo_Biotec.jpg
 +
|objectives=4x/0.13<br>10x/0.3 Ph1<br>20x/0.5 Ph1 DIC<br>40x/0.75 Ph2 DIC<br>100x/1.35-0.5 Oil Ph3 DIC
 +
|illumination=HBO lamp<br>Halogen lamp
 
}}
 
}}

Latest revision as of 16:27, 16 June 2014

Suitable for -.
Bioz12.jpg


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 227)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Olympus - BX 61, Upright stand, manual XY stage, motorized Z-stage, fluorescence, transmitted light with manual DIC, color and b/w acquisition
objectives
Olympus UPlanFl 4x 0.13
Olympus UPlanSApo 10x 0.4
Olympus UPlanFl 20x 0.5 Ph1
Olympus UPlanSApo 20x 0.85 Oil
Olympus UPlanFLN 40X 0.75 Ph2
Olympus UPlanSApo 60x 1.35 Oil
Olympus UPlanApo 100x 1.35 oil Ph3
Olympus UPlanSApo 40x 0.95
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
detection Coolsnap fx (1300x1030 pixel; 6,7x6,7μm; 12bit@20MHz)

Spot Insight QE Color (2048x2048 pixel; 7,4x7,4μm; 14bit@20MHz)
Olympus DP71 color (1360x1024, up to 4080x3072 by pixel shift; 6,45x6x45 µm)

reflectors DAPI : EX 365/10 ; BS 400 ; EM LP 420

GFP/FITC : EX 480/20 ; BS 495 ; EM 525/50
GFP/FITC : EX 470/40 ; BS 495 ; EM 525/50
Cy3 : EX 545/25 ; BS 565 ; EM 605/70
Cy5 : EX 620/60 ; BS 660 ; EM 700/75
TRITC : EX 540/20 ; BS 570 ; EM 590

features timelapse imaging, z-stack, Brightfield, Fluorescence
software MetaMorph

SPOT Basic/Advanced"MetaMorph SPOT Basic/Advanced" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 106553



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