WF Olympus upright, BIOTEC
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO22 |
| − | |system-name=Olympus | + | |system-name=WF Olympus upright, BIOTEC |
|type=Microscope | |type=Microscope | ||
|category=Wide-Field | |category=Wide-Field | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
| − | |building= | + | |building=BIOTEC |
| − | |room= | + | |room=227 |
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
| − | |Institute-logo= | + | |Institute-logo=Biotec logo.png |
|description=Our Olympus videomicroscope upright is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to the use of two possible cameras, a b/w Photometrics and a color Spot CCD. Timelapse, along with z-stacks can be performed with the use of motorized z-stage | |description=Our Olympus videomicroscope upright is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to the use of two possible cameras, a b/w Photometrics and a color Spot CCD. Timelapse, along with z-stacks can be performed with the use of motorized z-stage | ||
|applications=- | |applications=- | ||
|image=Bioz12.jpg | |image=Bioz12.jpg | ||
| − | |stand-name=Olympus - | + | |stand-name=Olympus - BX 61 |
|stand=Upright | |stand=Upright | ||
|microscope=manual XY stage, motorized Z-stage, fluorescence, transmitted light with manual DIC, color and b/w acquisition | |microscope=manual XY stage, motorized Z-stage, fluorescence, transmitted light with manual DIC, color and b/w acquisition | ||
| − | |obj1=Olympus | + | |obj1=Olympus UPlanFl 4x 0.13 |
| − | |obj2=Olympus UPlanSApo | + | |obj2=Olympus UPlanSApo 10x 0.4 |
| − | |obj3=Olympus | + | |obj3=Olympus UPlanFl 20x 0.5 Ph1 |
| − | |obj4=Olympus | + | |obj4=Olympus UPlanSApo 20x 0.85 Oil |
| − | |obj5=Olympus | + | |obj5=Olympus UPlanFLN 40X 0.75 Ph2 |
| − | |obj6=Olympus | + | |obj6=Olympus UPlanSApo 60x 1.35 Oil |
| + | |obj7=Olympus UPlanApo 100x 1.35 oil Ph3 | ||
| + | |obj8=Olympus UPlanSApo 40x 0.95 | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
| − | |detection=Coolsnap fx (1300x1030 pixel; 6,7x6,7μm; 12bit@20MHz) | + | |detection=Coolsnap fx (1300x1030 pixel; 6,7x6,7μm; 12bit@20MHz) <br> |
| − | Spot Insight QE Color (2048x2048 pixel; 7,4x7,4μm; | + | Spot Insight QE Color (2048x2048 pixel; 7,4x7,4μm; 14bit@20MHz) <br> |
| − | 14bit@20MHz) | + | Olympus DP71 color (1360x1024, up to 4080x3072 by pixel shift; 6,45x6x45 µm) |
| − | |reflectors=DAPI : EX 365/10 ; BS 400 ; EM LP 420<br>FITC : EX 480/20 ; BS 495 ; EM | + | |reflectors=DAPI : EX 365/10 ; BS 400 ; EM LP 420<br> |
| + | GFP/FITC : EX 480/20 ; BS 495 ; EM 525/50<br> | ||
| + | GFP/FITC : EX 470/40 ; BS 495 ; EM 525/50<br> | ||
| + | Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br> | ||
| + | Cy5 : EX 620/60 ; BS 660 ; EM 700/75<br> | ||
| + | TRITC : EX 540/20 ; BS 570 ; EM 590 | ||
|features=timelapse imaging, z-stack, Brightfield, Fluorescence | |features=timelapse imaging, z-stack, Brightfield, Fluorescence | ||
| − | |software= | + | |software=MetaMorph |
| + | SPOT Basic/Advanced | ||
|incubation=Not available | |incubation=Not available | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
| − | |inv= | + | |inv=106553 |
|facility_image=Imaging@Biotec.jpg | |facility_image=Imaging@Biotec.jpg | ||
|logo_image=Logo_Biotec.jpg | |logo_image=Logo_Biotec.jpg | ||
Latest revision as of 16:27, 16 June 2014
|
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Olympus - BX 61, Upright stand, manual XY stage, motorized Z-stage, fluorescence, transmitted light with manual DIC, color and b/w acquisition | ||
| objectives | |||
| illumination | |||
| detection | Coolsnap fx (1300x1030 pixel; 6,7x6,7μm; 12bit@20MHz) Spot Insight QE Color (2048x2048 pixel; 7,4x7,4μm; 14bit@20MHz) | ||
| reflectors | DAPI : EX 365/10 ; BS 400 ; EM LP 420 GFP/FITC : EX 480/20 ; BS 495 ; EM 525/50 | ||
| features | timelapse imaging, z-stack, Brightfield, Fluorescence | ||
| software | MetaMorph
SPOT Basic/Advanced"MetaMorph SPOT Basic/Advanced" cannot be used as a page name in this wiki. | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | 106553 |
