Zeiss ApoTome - DZNE W1

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{{Equipment
 
{{Equipment
|system-number=DZNE-01
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|system-number=DZNE-W1
 
|system-name=Zeiss ApoTome
 
|system-name=Zeiss ApoTome
 
|type=Microscope
 
|type=Microscope
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|Institute-logo=Logo-DZNE.jpg
 
|Institute-logo=Logo-DZNE.jpg
 
|description=It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
 
|description=It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
 
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|applications=fixed samples, fluorescently labeled samples (structured illumination possible), histologically fixed samples (no strucutred illumination), big samples
For imaging your histologically stained samples the system now also features a coloured camera.
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|applications=
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*fixed samples
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*fluorescently labeled samples (structured illumination possible)
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*histologically fixed samples (no strucutred illumination)
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|image=Zeiss ApoTome - DZNE 01.jpg
 
|image=Zeiss ApoTome - DZNE 01.jpg
 
|stand-name=Zeiss - Axio Imager.M2
 
|stand-name=Zeiss - Axio Imager.M2
 
|stand=Upright
 
|stand=Upright
|microscope=manual XY stage, motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
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|microscope=motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
|obj1=Zeiss EC Plan Neofluar 2.5x 0.075
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|obj1=Zeiss EC Plan Neofluar 5x 0.16
 
|obj2=Zeiss Plan-Apochromat 10x 0.45
 
|obj2=Zeiss Plan-Apochromat 10x 0.45
 
|obj3=Zeiss Plan-Apochromat 20x 0.8
 
|obj3=Zeiss Plan-Apochromat 20x 0.8
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|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
|detection=
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|detection=<br>
 
* Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
 
* Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
* Axiocam 503 color (1936*1460, 4,54*4,54µm)
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|reflectors=<br>
|reflectors=FS 49    ''(DAPI)''    : EX BP G365; BS 395; EM  BP 445/50<br>
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*FS 49    ''(DAPI)''    : EX BP G365; BS 395; EM  BP 445/50<br>
FS 38 HE ''(GFP)''      : EX BP 470/40; BS 495; EM BP 525/50 <br>
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*FS 38 HE ''(GFP)''      : EX BP 470/40; BS 495; EM BP 525/50 <br>
FS 20  ''(Rhodamine)'' : EX BP 546/12; BS 560; BP 575-640  <br>
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*FS 20  ''(Rhodamine)'' : EX BP 546/12; BS 560; BP 575-640  <br>
FS 50    ''(Cy5)''      : EX BP 640/30 ; BS 660 ; EM 690/50 <br>
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*FS 50    ''(Cy5)''      : EX BP 640/30 ; BS 660 ; EM 690/50 <br>
 
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<br>
FS Analysator for DIC and Transmission
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*FS Analysator for DIC and Transmission
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, histollogical imaging
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|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, tile scans, multi position experiments, complex experiments via Experiment Designer, optical sectioning
 
|software=ZEN Blue 2012
 
|software=ZEN Blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=0
 
|inv=0
 
}}
 
}}

Latest revision as of 10:00, 3 August 2016

Suitable for fixed samples, fluorescently labeled samples (structured illumination possible), histologically fixed samples (no strucutred illumination), big samples.
Zeiss ApoTome - DZNE 01.jpg


[edit] Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

[edit] Details

microscope Zeiss - Axio Imager.M2, Upright stand, motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
objectives
Zeiss EC Plan Neofluar 5x 0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 100x 1.3 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection
  • Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
reflectors
  • FS 49 (DAPI)  : EX BP G365; BS 395; EM BP 445/50
  • FS 38 HE (GFP)  : EX BP 470/40; BS 495; EM BP 525/50
  • FS 20 (Rhodamine) : EX BP 546/12; BS 560; BP 575-640
  • FS 50 (Cy5)  : EX BP 640/30 ; BS 660 ; EM 690/50


  • FS Analysator for DIC and Transmission
features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, tile scans, multi position experiments, complex experiments via Experiment Designer, optical sectioning
software ZEN Blue 2012
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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