Zeiss ApoTome - DZNE W1

From BioDIP
(Difference between revisions)
Jump to: navigation, search
(new equipment dzne)
 
 
(30 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{Equipment
 
{{Equipment
|system-number=BIO02
+
|system-number=DZNE-W1
|system-name=ApoTome1 3rd floor, upr., CRTD
+
|system-name=Zeiss ApoTome
 
|type=Microscope
 
|type=Microscope
|category=Wide-Field
+
|category=Structured Illumination
|facility=Imaging@Biotec
+
|facility=DZNE - Imaging Platform
|building=Biotec
+
|building=DZNE
|room=3.235
+
|room=2.08
|Facility-logo=Imaging@Biotec.jpg
+
|Facility-logo=DZNE-Imaging-logo2-v6.png
|Institute-logo=CRTD logo.PNG
+
|Institute-logo=Logo-DZNE.jpg
|description=It is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
+
|description=It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
|applications=-
+
|applications=fixed samples, fluorescently labeled samples (structured illumination possible), histologically fixed samples (no strucutred illumination), big samples
|image=ApoTome1 3rd floor, upr., CRTD.jpg
+
|image=Zeiss ApoTome - DZNE 01.jpg
|stand-name=Zeiss - Axio Imager
+
|stand-name=Zeiss - Axio Imager.M2
 
|stand=Upright
 
|stand=Upright
|microscope=motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (Apotome), color acquisition for stained histological samples
+
|microscope=motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
|obj1=Zeiss Plan-Apochromat 10x 0.45
+
|obj1=Zeiss EC Plan Neofluar 5x 0.16
|obj2=Zeiss Plan-Apochromat 20x 0.8
+
|obj2=Zeiss Plan-Apochromat 10x 0.45
|obj3=Zeiss Plan-Apochromat 40x 0,95 korr.
+
|obj3=Zeiss Plan-Apochromat 20x 0.8
 
|obj4=Zeiss Plan-Apochromat 40x 0,95 korr.
 
|obj4=Zeiss Plan-Apochromat 40x 0,95 korr.
|obj5=Zeiss EC Plan Neofluar 2.5x 0.075
+
|obj5=Zeiss EC Plan-Neofluar 100x 1.3 Oil
|obj6=Zeiss Achroplan 40x 0.8 W
+
|obj7=Zeiss EC Plan-Neofluar 40x 1.3 Oil
+
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w<br>
+
|detection=<br>
Axiocam MRc (1388*1040 pixel, 6,45*6,45µm) - color
+
* Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
|reflectors=FS 49: EX BP G365; BS 395; EM  BP 445/50 (Dapi)<br>
+
|reflectors=<br>
FS HC-FITC: EX BP 482/35 (HE); BS 505 (HE); EM BP 536/40 (HE); (GFP)<br>
+
*FS 49   ''(DAPI)''    : EX BP G365; BS 395; EM  BP 445/50<br>
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
+
*FS 38 HE ''(GFP)''      : EX BP 470/40; BS 495; EM BP 525/50 <br>
FS Cy5 HC: EX BP 640/30 ; BS 660 ; EM 690/50; (Cy5)<br>
+
*FS 20  ''(Rhodamine)'' : EX BP 546/12; BS 560; BP 575-640  <br>
FS 24: EX BP 485/20 - BP 578/14; BS 500 - LP 600; EM 527/25 - LP 610; (GFP-RFP)<br>
+
*FS 50    ''(Cy5)''      : EX BP 640/30 ; BS 660 ; EM 690/50 <br>
FS Analysator for DIC and Transmission
+
<br>
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
+
*FS Analysator for DIC and Transmission
|software=Axiovision
+
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, tile scans, multi position experiments, complex experiments via Experiment Designer, optical sectioning
 +
|software=ZEN Blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=0
 
|inv=0
 
}}
 
}}

Latest revision as of 10:00, 3 August 2016

Suitable for fixed samples, fluorescently labeled samples (structured illumination possible), histologically fixed samples (no strucutred illumination), big samples.
Zeiss ApoTome - DZNE 01.jpg


[edit] Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

[edit] Details

microscope Zeiss - Axio Imager.M2, Upright stand, motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
objectives
Zeiss EC Plan Neofluar 5x 0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 100x 1.3 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection
  • Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
reflectors
  • FS 49 (DAPI)  : EX BP G365; BS 395; EM BP 445/50
  • FS 38 HE (GFP)  : EX BP 470/40; BS 495; EM BP 525/50
  • FS 20 (Rhodamine) : EX BP 546/12; BS 560; BP 575-640
  • FS 50 (Cy5)  : EX BP 640/30 ; BS 660 ; EM 690/50


  • FS Analysator for DIC and Transmission
features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, tile scans, multi position experiments, complex experiments via Experiment Designer, optical sectioning
software ZEN Blue 2012
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



Personal tools