Zeiss ApoTome - DZNE W1

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m (Whites moved page Zeiss ApoTome - DZNE 01 to Zeiss ApoTome - DZNE W1: name change)
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|stand-name=Zeiss - Axio Imager.M2
 
|stand-name=Zeiss - Axio Imager.M2
 
|stand=Upright
 
|stand=Upright
|microscope=manual XY stage, motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
+
|microscope=motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
|obj1=Zeiss EC Plan Neofluar 2.5x 0.075
+
|obj1=Zeiss EC Plan Neofluar 5x 0.16
 
|obj2=Zeiss Plan-Apochromat 10x 0.45
 
|obj2=Zeiss Plan-Apochromat 10x 0.45
 
|obj3=Zeiss Plan-Apochromat 20x 0.8
 
|obj3=Zeiss Plan-Apochromat 20x 0.8
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|detection=<br>
 
|detection=<br>
 
* Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
 
* Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
* Axiocam 503 color (1936*1460, 4,54*4,54µm)
 
 
|reflectors=<br>
 
|reflectors=<br>
 
*FS 49    ''(DAPI)''    : EX BP G365; BS 395; EM  BP 445/50<br>
 
*FS 49    ''(DAPI)''    : EX BP G365; BS 395; EM  BP 445/50<br>
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*timelapse
 
*timelapse
 
*z-stack
 
*z-stack
 +
*tile scans
 +
*multi position experiments
 +
*complex experiment via Experiment Designer
 
*optical sectioning
 
*optical sectioning
 
*histollogical imaging
 
*histollogical imaging

Revision as of 09:53, 3 August 2016

For imaging your histologically stained samples the system now also features a coloured camera."It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome. For imaging your histologically stained samples the system now also features a coloured camera." cannot be used as a page name in this wiki.

Suitable for
  • fixed samples
  • fluorescently labeled samples (structured illumination possible)
  • histologically fixed samples (no strucutred illumination)"<br />
  • fixed samples
  • fluorescently labeled samples (structured illumination possible)
  • histologically fixed samples (no strucutred illumination)" cannot be used as a page name in this wiki..
Zeiss ApoTome - DZNE 01.jpg


Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope Zeiss - Axio Imager.M2, Upright stand, motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
objectives
Zeiss EC Plan Neofluar 5x 0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 100x 1.3 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection
  • Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
reflectors
  • FS 49 (DAPI)  : EX BP G365; BS 395; EM BP 445/50
  • FS 38 HE (GFP)  : EX BP 470/40; BS 495; EM BP 525/50
  • FS 20 (Rhodamine) : EX BP 546/12; BS 560; BP 575-640
  • FS 50 (Cy5)  : EX BP 640/30 ; BS 660 ; EM 690/50


  • FS Analysator for DIC and Transmission
features
  • sequential imaging
  • Brightfield and Fluorescence overlay
  • timelapse
  • z-stack
  • tile scans
  • multi position experiments
  • complex experiment via Experiment Designer
  • optical sectioning
  • histollogical imaging"<br />
  • sequential imaging
  • Brightfield and Fluorescence overlay
  • timelapse
  • z-stack
  • tile scans
  • multi position experiments
  • complex experiment via Experiment Designer
  • optical sectioning
  • histollogical imaging" cannot be used as a page name in this wiki.
software ZEN Blue 2012
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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