Zeiss ApoTome - DZNE W1

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|Institute-logo=Logo-DZNE.jpg
 
|Institute-logo=Logo-DZNE.jpg
 
|description=It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
 
|description=It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
 
''For imaging your histologically stained samples the system now also features a coloured camera.''
 
 
|applications=<br>
 
|applications=<br>
 
*fixed samples
 
*fixed samples
 
*fluorescently labeled samples (structured illumination possible)
 
*fluorescently labeled samples (structured illumination possible)
 
*histologically fixed samples (no strucutred illumination)
 
*histologically fixed samples (no strucutred illumination)
 +
*big samples
 
|image=Zeiss ApoTome - DZNE 01.jpg
 
|image=Zeiss ApoTome - DZNE 01.jpg
 
|stand-name=Zeiss - Axio Imager.M2
 
|stand-name=Zeiss - Axio Imager.M2
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*complex experiment via Experiment Designer
 
*complex experiment via Experiment Designer
 
*optical sectioning
 
*optical sectioning
*histollogical imaging
+
*histological imaging
 
|software=ZEN Blue 2012
 
|software=ZEN Blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=0
 
|inv=0
 
}}
 
}}

Revision as of 09:54, 3 August 2016

Suitable for
  • fixed samples
  • fluorescently labeled samples (structured illumination possible)
  • histologically fixed samples (no strucutred illumination)
  • big samples"<br />
  • fixed samples
  • fluorescently labeled samples (structured illumination possible)
  • histologically fixed samples (no strucutred illumination)
  • big samples" cannot be used as a page name in this wiki..
Zeiss ApoTome - DZNE 01.jpg


Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope Zeiss - Axio Imager.M2, Upright stand, motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
objectives
Zeiss EC Plan Neofluar 5x 0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 100x 1.3 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection
  • Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
reflectors
  • FS 49 (DAPI)  : EX BP G365; BS 395; EM BP 445/50
  • FS 38 HE (GFP)  : EX BP 470/40; BS 495; EM BP 525/50
  • FS 20 (Rhodamine) : EX BP 546/12; BS 560; BP 575-640
  • FS 50 (Cy5)  : EX BP 640/30 ; BS 660 ; EM 690/50


  • FS Analysator for DIC and Transmission
features
  • sequential imaging
  • Brightfield and Fluorescence overlay
  • timelapse
  • z-stack
  • tile scans
  • multi position experiments
  • complex experiment via Experiment Designer
  • optical sectioning
  • histological imaging"<br />
  • sequential imaging
  • Brightfield and Fluorescence overlay
  • timelapse
  • z-stack
  • tile scans
  • multi position experiments
  • complex experiment via Experiment Designer
  • optical sectioning
  • histological imaging" cannot be used as a page name in this wiki.
software ZEN Blue 2012
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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