Zeiss ApoTome - DZNE W1

From BioDIP
Revision as of 09:57, 3 August 2016 by Whites (Talk | contribs)

Jump to: navigation, search
Suitable for
  • fixed samples
  • fluorescently labeled samples (structured illumination possible)
  • histologically fixed samples (no strucutred illumination)
  • big samples"<br />
  • fixed samples
  • fluorescently labeled samples (structured illumination possible)
  • histologically fixed samples (no strucutred illumination)
  • big samples" cannot be used as a page name in this wiki..
Zeiss ApoTome - DZNE 01.jpg


Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope Zeiss - Axio Imager.M2, Upright stand, motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
objectives
Zeiss EC Plan Neofluar 5x 0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 100x 1.3 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection
  • Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
reflectors
  • FS 49 (DAPI)  : EX BP G365; BS 395; EM BP 445/50
  • FS 38 HE (GFP)  : EX BP 470/40; BS 495; EM BP 525/50
  • FS 20 (Rhodamine) : EX BP 546/12; BS 560; BP 575-640
  • FS 50 (Cy5)  : EX BP 640/30 ; BS 660 ; EM 690/50


  • FS Analysator for DIC and Transmission
features *sequential imaging
  • Brightfield and Fluorescence overlay
  • timelapse
  • z-stack
  • tile scans
  • multi position experiments
  • complex experiment via Experiment Designer
  • optical sectioning"*sequential imaging
  • Brightfield and Fluorescence overlay
  • timelapse
  • z-stack
  • tile scans
  • multi position experiments
  • complex experiment via Experiment Designer
  • optical sectioning" cannot be used as a page name in this wiki.
software ZEN Blue 2012
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



Personal tools