Zeiss LSM700 - DZNE C1

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{{Equipment
 
{{Equipment
|system-number=MPI-CZ5
+
|system-number=DZNE-C1
|system-name=Zeiss LSM 700 inverted
+
|system-name=Zeiss LSM700
 
|type=Microscope
 
|type=Microscope
 
|category=Laser Scanning Confocal
 
|category=Laser Scanning Confocal
|facility=MPI-CBG LMF
+
|facility=DZNE - Imaging Platform
|building=MPI-CBG
+
|building=DZNE
|room=038a
+
|room=2.08
 +
|Facility-logo=DZNE-Imaging-logo2-v6.png
 +
|Institute-logo=Logo-DZNE.jpg
 
|description=Routine confocal workhorse for simple, standard and tile scan jobs.
 
|description=Routine confocal workhorse for simple, standard and tile scan jobs.
 
It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|applications=2D images, z-stacks, time lapse, bleaching and tile scans
 
|applications=2D images, z-stacks, time lapse, bleaching and tile scans
|image=Zeiss LSM 700.jpg
+
|image=DZNE_DUO-system.jpg
|stand-name=Zeiss - Axio Observer
+
|stand-name=Zeiss - Axio Observer.Z1
 
|stand=Inverted
 
|stand=Inverted
|microscope=-
+
|microscope=motorized, motorized xy scanning stage, motorized z-drive
|obj1=Zeiss Plan-Apochromat 10x 0.45
+
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
|obj2=Zeiss Plan-Apochromat 20x 0.8
+
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
|obj3=Zeiss Plan-Neofluar 25x 0.8 Imm Corr
+
|obj3=Zeiss Plan-Apochromat 20x 0.8
|obj4=Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
+
|obj4=Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
 +
|obj5=Zeiss Plan-Apochromat 40x 0,95 korr.
 +
|obj6=Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
 
|ill1=Laser Diode 405 nm
 
|ill1=Laser Diode 405 nm
 
|ill2=Laser DPSS 488 nm
 
|ill2=Laser DPSS 488 nm
|ill3=Laser DPSS 555nm
+
|ill3=Laser DPSS 555 nm
|ill4=Laser DPSS 639nm
+
|ill4=Laser Diode 639 nm
 
|ill5=Transmitted Light (Halogen)
 
|ill5=Transmitted Light (Halogen)
 
|ill6=Fluorescence (Metal Halide)
 
|ill6=Fluorescence (Metal Halide)
 
|detection=2 PMTs<br>
 
|detection=2 PMTs<br>
 
No transmission detector
 
No transmission detector
|reflectors=Dapi : EX 350/50 ; BS 395 ; EM LP 420 <br>
+
|reflectors=Dapi : EX G 365 ; BS 395 ; EM 445/50 <br>
FITC : EX 480/30 ; BS 505 ; EM 535/40 <br>
+
eGFP : EX 470/40 ; BS 495 ; EM 525/50 <br>
DsRed : EX 545/10 ; BS 555 ; EM 600/70 <br>
+
Cy3  : EX 545/25 ; BS 570 ; EM 605/70 <br>
GFP/RFP : EX ; BS ; EM
+
Cy5  : EX 640/30 ; BS 660 ; EM 690/50 <br>
|features=-
+
|features=<br>
|software=ZEN 2010
+
* number of different stage inserts to meet a variety of samples:
|incubation=Not available
+
- universal holder: for 1 slide or 1 dish <br>
|link0=http://www.zeiss.de/C12567BE0045ACF1/Contents-Frame/A22821A1BDCAAE49C12574E80030638E
+
- multi slide holder: for up to 4 slides <br>
|inv=0   10.1.145.33
+
- multiwell holder
 +
* fully integrated, dark incubation chamber
 +
<br>
 +
|software=ZEN 2012
 +
|incubation=Cage incubator (T+CO2)
 +
|link2=https://docs.google.com/drawings/d/1Ystue84FdcWcYfmU2_-jdYKEwZazYzb5OdUWU1IWyik/pub?w=960&h=720
 +
|inv=0
 
}}
 
}}

Latest revision as of 16:31, 28 January 2016

It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with."Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with." cannot be used as a page name in this wiki.

Suitable for 2D images, z-stacks, time lapse, bleaching and tile scans.
DZNE DUO-system.jpg


[edit] Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, motorized, motorized xy scanning stage, motorized z-drive
objectives
Zeiss EC Plan-Neofluar 5x 0.16 Ph1
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss Plan-Apochromat 20x 0.8
Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 555 nm
Laser Diode 639 nm
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection 2 PMTs

No transmission detector

reflectors Dapi : EX G 365 ; BS 395 ; EM 445/50

eGFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3  : EX 545/25 ; BS 570 ; EM 605/70
Cy5  : EX 640/30 ; BS 660 ; EM 690/50

features
  • number of different stage inserts to meet a variety of samples:

- universal holder: for 1 slide or 1 dish
- multi slide holder: for up to 4 slides
- multiwell holder

  • fully integrated, dark incubation chamber


"<br />

  • number of different stage inserts to meet a variety of samples:

- universal holder: for 1 slide or 1 dish <br /> - multi slide holder: for up to 4 slides <br /> - multiwell holder

  • fully integrated, dark incubation chamber

<br />" cannot be used as a page name in this wiki.

software ZEN 2012
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
inv.nr. 0



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